Association Of Long Non-coding RNA MEG3 Polymorphisms And Risk Of Prostate Cnacer In Suwan Han Population

Objectives:Maternally expressed gene 3(MEG3)is the first Long non-coding RNA(Lnc RNA)identified as tumor suppressor.Some single nucleotide polymorphisms(SNPs)in MEG3 have been demonstrated to have influence on the cancer risk such as lung cancer,liver cancer and so on.But there was no study on the association between MEG3 polymorphisms and the risk of prostate cancer in Chinese Han population.The objects of this study were to evaluate the association of environmental factors and MEG3 polymorphism in prostate cancer susceptibility.Methods:This study recruited of 165 prostate cancer cases and 200 control subjects from the Affiliated Zhongda Hospital of Southeast University.PCa patients were diagnosed between July 2017 and December 2019 and were pathologically proven to have prostate adenocarcinoma after biopsy in the Affiliated Zhongda Hospital of Southeast University.The control group was age-matched,and the subjects were healthy checkup examinees without cancer history and were collected in the same period.All the patients were southern Chinese Han population.After informed consent was obtained,2ml of peripheral blood sample was collected and each subject was asked to finish a questionnaire.Two MEG3 SNPs(rs11627993C>T rs7158663 A>G))were genotyped in a case-control study by a Real-Time Polymerase Chain Reaction(PCR).The odds ratios(ORs)and 95%confidence intervals(CIs)was used to estimate the strength of association.X~2 test or student-t test were used to analyze the risk factors of prostate cancer by comparing the demographic characteristics and the distribution of MEG3 gene types between the cases and the control group.Odds ratios(ORs)representing relative risk were calculated by univariate and multivariate logistic regression.Results:Baseline characteristics were similar between cases and controls except that the frequency of relatives with cancer from the case group was higher,compared to nonrelatives(28.5%vs16%,P<0.01).For MEG3 rs11627993,the frequencies of the three genotypes were 14.55%(CC),51.51%(CA)and 33.94%(AA)in the case group,and 13.50%(CC),47%(CA)and 39.50%(AA)in the control group,respectively,with no significant difference between the two groups(P=0.62).For MEG3 rs7158663,the frequencies of the three genotypes in the case group were 7.88%(AA),32.73%(AG)and 59.39%(GG),respectively,and those in the control group were 5.50%(AA),39.00%(AG)and 55.50%(GG),respectively,with no significant difference between the two groups(P=0.34).No statistically significant differences were found in the allele or genotype distributions of the MEG3 rs11627993 C>A and rs7158663 A>G polymorphisms among cases or healthy control subjects.Further stratified analysis detected no significant association,either.Conclusion:The MEG3 polymorphisms(rs11627993 C>A and rs7158663 A>G)may have no influence on the susceptibility of the prostate cancer.More potentially functionally polymorphisms in MEG3 need to be studied in a larger series based on multi-hospitals.