A Gene Expression Profiling Study Of Ossification Of Ligamentun Flavum

Objective To investigate the genes abnormally expressed in TOLF and their related biological processes and metabolic pathways through differential expression analysis and enrichment analysis by high-throughput sequencing of peripheral blood samples from TOLF patients and healthy people using human whole genome expression profile chip technology.To explore the mechanism of osteogenesis in OLF cells at the molecular level,and to provide molecular theoretical basis for the pathogenesis of OLF in clinical practice.Methods From July to October 2020,5 patients with thoracic OLF and 5control subjects diagnosed in the General Hospital of Ningxia Medical University were selected,and 4ml of fasting peripheral venous blood was collected.The total RNA of samples was extracted with Trizol reagent,and the purity and quality of RNA were detected.The m RNAs significantly differentially expressed between the groups were found by Whole genome expression profiling chip technology,Then,the Gene Ontology(GO)and KEGG bioinformatics analysis were performed on the differentially expressed m RNAs by R software.Finally,several differentially expressed m RNAs were selected for q RT-PCR verification using the quantitative PCR kit.Results 1.High-throughput sequencing results showed that a total of 1088 differentially expressed m RNAs were up-regulated by 149 and down-regulated by 939.The reliability of the sequencing results was confirmed by q RT-PCR.GO enrichment analysis indicates that the difference of genes involved in biological processes,such as vascular glycoprotein metabolism,collaborative maintain fibroblast growth factor and other molecular function,in the cytoplasm and cell membrane,and other cellular components significantly expression,KEGG analysis showed differences in gene enrichment to(MAPK signal pathway,ECM signaling pathways,PI3K-Akt signaling pathways)and other signaling pathways.Conclusion1.There are differences in gene expression profiles in peripheral blood between TOLF patients and healthy people2.VWF,VEGFB,MMP9 and other basic cytokines may play an important role in the pathogenesis of TOLF.3.Cell adhesion,autophagy and other processes may play an important role in the ossification of LF.4.MAPK signaling pathway,ECM signaling pathway and PI3K-Akt signaling pathway may be important cellular pathways in the pathogenesis of TOLF.