Expression And Function Of EIF3B In Squamous Cell Carcinoma Of The Tongue

Cancer is becoming the main cause of death in all countries,and its incidence is increasing year by year,which brings huge challenges to people’s health and national finances.Oral cancer is the sixth most common malignant tumor.Squamous cell carcinoma of the tongue(TSCC)is the main type of malignant tumors of the head and neck.In recent years,with the rapid development of medical technology,the treatment rate of tumors has been greatly improved.However,the 5-year survival rate still fluctuates around 50%.EIF3 B is a major scaffold protein of EIF3 which is the largest translation initiation factor.It participates in a variety of important cellular activities and has been related to the progression of a variety of tumors.Objective:To explore the expression and function of EIF3 B in TSCC.Method:Part Ⅰ: The whole protein sequencing was performed on 10 pairs patients’ cancerous tissue of TSCC and paracancerous tissues,and WGCNA(weighted gene co-expression network analysis)was performed on the sequencing results.After prognostic analysis,seven differential genes were verified.Then EIF3 B was screened out for further functional verification by HPA(human protein atlas)and Oncomine database analysis.Part Ⅱ: The m RNA expression of EIF3 B in carcinoma tissue and paracancerous tissues of TSCC was verified by q RT-PCR(quantitative real-time polymerase chain reaction).Immunohistochemical assay was used to verify the protein expression of EIF3 B in the carcinoma and paracancerous tissues of TSCC.Part Ⅲ: CAL 27 cell line of TSCC was infected with lentivirus LV-e IF3 B RNAi,and CAL 27 cell line of stable knockdown EIF3 B was constructed.q RT-PCR and WB were used to verify the level of knockdown.The effect of knockdown EIF3 B on the function of CAL 27 cell line of TSCC was verified by cell proliferation experiments,plate cloning experiments,Transwell chamber experiments,and scratch assay.The expression of key proteins in cell line of knockdown EIF3 b was detected by WB and q RT-PCR.Result:Part Ⅰ: Seven key genes(NDUFB3,RPL12,EIF3 B,DDOST,PFDN2,HNRNPH1,and SEC61G)were found to be related to the development and prognosis of TSCC by WGCNA.Four hub genes(EIF3B,DDOST,PFDN2,SEC61G)were highly expressed in head and neck cancer tissues in different degrees,and the prognosis was significantly different in HPA and Oncomine database.Part Ⅱ: The results of q RT-PCR showed that EIF3 B,DDOST,PFDN2,SEC61 G were up-regulated in TSCC compared with paracancerous tissues,and the difference was statistically significant(P<0.05).The results of immunohistochemistry showed that the expression of EIF3 B in the tissue of TSCC was higher than that in paracancerous tissues.Part Ⅲ: Compared with the non-transfection group,the results of CCK-8proliferation test,plate cloning experiments,Transwell chamber experiments and scratch assay in the knockdown group showed that the proliferation,migration and invasion abilities of cells were decreased,and the difference was statistically significant(P<0.05).The results in the knockdown group showed that the expression of E-cadherin was increased and the expression of N-cadherin was decreased in CAL 27 cell line of TSCC by q RT-PCR and WB.The difference was statistically significant(P < 0.05).Conclusion:The expression of EIF3 B in TSCC was higher than that in paracancerous tissues.The knockdown of EIF3 B has a significant impact on the proliferation,migration and invasion abilities of the CAL 27 cell line of TSCC,and has a certain impact on the EMT(epithelial mesenchymal transition)process.