Expression Of FOXP3 And IL-17A In Cervical Tissues Of Patients With High-risk HPV Infection-related Cervical Intraepithelial Lesions And Cervical Cancer

Objective: Cervical cancer is one of the most common gynecological malignancies.In recent years,its incidence has been increasing year by year and becoming younger,and it has become one of the major diseases that seriously threaten the health and safety of women in our country.To explore the role of regulatory T cells(Treg cells)and T helper cells(Th17 cells)in the occurrence and development of cervical intraepithelial lesions and cervical cancer related to HPV infection.Methods: Selected patients with high-risk HPV infection in the gynecology department of the Fourth Hospital of Hebei Medical University from September 2018 to December 2020 as the research objects.According to the pathological diagnosis of cervical biopsy,they were divided into normal group(NSIL group)and low-grade intraepithelial lesion group(LSIL group).,High-grade intraepithelial lesion group(HSIL group)and cervical cancer(CC)group;according to whether there is high-risk HPV16 or HPV18 infection,it is divided into key groups(with HPV16 or HPV18 infection)and other groups(except HPV16 or HPV18 other high-risk HPV infection).The Department of Histology and Embryology of Hebei Medical University was entrusted to detect the expression of FOXP3 and IL-17 A in the cervical tissues of the above patients by immunohistochemistry.Results: 1.The older the subjects included in the study,the more births,the higher the proportion of high-risk HPV16 and 18 infections,and the larger the proportion of unconventional gynecological examinations,the greater the incidence of cervical intraepithelial lesions and cervical cancer.2.The expression levels of FOXP3 and IL-17 A in the high-risk HPV16 and HPV18 infection groups are higher than other high-risk HPV infection groups,and the difference is statistically significant(Z=-6.052,p<0.05;Z=-5.217;P <0.05).3.The expression levels of FOXP3 and IL-17 A in cervical intraepithelial lesions and cervical cancer tissues were higher than those in the normal group,and the difference was statistically significant(Z=-6.450,P <0.05;Z=-7.030;P <0.05).With the aggravation of cervical lesions,the expression levels of FOXP3 and IL-17A showed an upward trend.4.Compared with the NSIL group(1.262±0.434),Th17 in the LSIL group(0.790±0.242,P<0.05),HSIL group(0.807±0.237,P<0.05)and CC group(0.343±0.109,P<0.05)/Treg ratio decreased;CC group was compared with NSIL group,LSIL group,HSIL group,the difference was statistically significant(P <0.05);but the difference between LSIL group and HSIL group was not statistically significant(P >0.05).Conclusions: 1.The older the age,the greater the number of births,the presence of high-risk HPV16 and 18 infections,and irregular gynecological physical examinations can increase the risk of cervical intraepithelial lesions and cervical cancer in people with high-risk HPV infections.2.HPV16 or HPV18 infection-related cervical intraepithelial lesions and cervical cancer patients have higher expression levels of Treg cells and Th17 cells.3.In high-risk HPV infection-related cervical lesions and cervical cancer tissues,the expression levels of Treg cells and Th17 cells increase with the severity of cervical lesions;the Th17/Treg ratio of cervical intraepithelial lesions and cervical cancer groups is lower than that of the normal group.4.This study suggests that Treg cells and Th17 cells are expected to become targets for cervical cancer immunotherapy.