Study On The Difference Distribution Of The Active Components Of Salvia Miltiorrhiza And The Metabolic Pathways Of Phenolic Acids

Salvia miltiorrhiza Bunge,as a traditional natural medicinal plant in China,has thousands of years of medicinal history and remarkable medicinal activity.It is now used as a raw material for many traditional Chinese medicines,proprietary Chinese medicines and chemical medicines,and is mainly used in the treatment of cardiovascular diseases.The typical active natural products in medicinal salvia miltiorrhiza were studied in this study.UPLC-Q-TOF MS combined with non-target metabolomics analysis methods were used to determine the five salvia miltiorrhiza plants.Six typical natural products with significant differences in each tissue(leaf,stem bark,stem xylem,root bark,root xylem)are used as chemical markers for distinguishing the quality of Salvia miltiorrhiza tissues,namely Salvianolic acid B,Lithospermic acid,Rosemaric acid,Caffeic acid,Tanshinone ⅡB and Cryptotanshinone.Combined with the third-generation Pac Bio Sequel sequencing system and the secondgeneration Illumina Hi Seq sequencing,the transcriptome sequencing of the five tissues of Salvia miltiorrhiza with 76252 transcripts was obtained.Combining metabolomics and bioinformatics analysis,11 laccase genes that are highly likely to participate in salvianolic acid B synthesis were founded.Finally,3 highly expressed candidate genes were identified for heterologous expression function verification.A heterologous expression system of Salvia miltiorrhiza-Aspergillus oryzae was constructed,and the concentration of rosmarinic acid in the substrate was observed to decrease in the fermentation experiment with added substrate.For the first time,MALDI-MSI mass spectrometry imaging methods were used in the research of medicinal salvia miltiorrhiza,and eight typical compounds were obtained,including caffeic acid,ferulic acid,lithospermic acid,salvianolic acid B,tanshinoneⅡA,cryptotanshinone,tanshinone ⅡB and dihydrotanshinone I The spatial location of the typical compounds in the roots in the roots,and significant regional differences were obtained by K-means cluster analysis,and the spatial distribution patterns of 64 ions with typical regional distribution characteristics were obtained using co-localization analysis.This proves the feasibility of using MALDI for the research of salvianolic acids and tanshinone compounds,and provides data support for the synthesis,accumulation and transportation of salvia miltiorrhiza natural products.The purpose of this study is to explain the metabolic pathways of natural active compounds of Salvia miltiorrhiza.Based on cutting-edge equipment and analysis methods,multi-omics and multi-dimensional research methods are provided to provide new ideas for the research of natural medicinal plants.As a treasure of Chinese culture,Chinese medicine will continue to make great contributions to the cause of human health by taking advantage of the rapid development of life sciences.