Expression And Role Of TRAP1 In Hepatocellular Carcinoma

Objective: 1 To detect the expression of TRAP1 in human hepatocellular carcinoma(HCC)tissues and normal liver tissues.2 To the expression level of TRAP1 in normal liver cells LO2(LO2 group),liver cancer cells Hep G2(Hep G2 group)and transfection group(reduced by down-regulating the expression of TRAP1 in Hep G2 cells)and the proliferation and apoptosis ability of the three groups of cells to find therapeutic target that has specific killing effect on liver cancer cells without obvious side effects,and provides relevant theoretical basis for promoting more effective treatment of hepatocellular carcinoma.Methods: 1 Immunohistochemical method: To detect the expression level of TRAP1 in human HCC tissues and normal tissues adjacent to cancer.2 Cell experiment grouping: divide the cells into three groups: LO2 group,Hep G2 group and transfection group;use MTT method to detect cell proliferation;use Western Blot and q RT-PCR(Quantitative Real-time PCR)methods to detect TRAP1 and Caspase3 Protein and m RNA levels.Results: 1 Immunohistochemical method: The expression level of TRAP1 in human HCC tissues is higher than that in non-tumor tissues adjacent to cancer,and the difference is significant(P<0.05).The expression level of TRAP1 is related to its clinical stage,and the positive rate of TRAP1 is higher in patients with tumor stages III and IV.(P<0.05).2q RT-PCR and Western Blot: The expression of TRAP1 m RNA and protein in the LO2 group was lower than that of the Hep G2 group(P<0.05);the expression of TRAP1 m RNA and protein in the transfection group was lower than that in the Hep G2 group(P<0.05),but still high In the LO2 group;the m RNA and protein levels of Caspase3 in the LO2 group were higher than those in the Hep G2 group(P<0.05),and the m RNA and protein content of the Caspase3 in the transfection group were higher than those in the Hep G2 and LO2 groups,the difference was significant(P<0.05);Hep G2 was inhibited The level of TRAP1 leads to the up-regulation of Caspase3,thereby inducing cell apoptosis.3 MTT experiment: The proliferation level of the Hep G2 group was higher than that of the LO2 group and the transfection group,and the transfection group was higher than that of the LO2 group,the difference was significant(P<0.05).Conclusion:1 TRAP1 is highly expressed in human HCC tissues,and low in normal tissues adjacent to cancer.Among them,the expression level in cancer stages III and IV is higher than that in stages I and II.2 Inhibiting the level of TRAP1 in Hep G2 cells can reduce cell proliferation and up-regulate Caspase3,thereby promoting cell apoptosis,indicating that inhibiting its expression level may play a role in hindering the progress of HCC.