Study On The Role And Mechanism Of MiR-195 Targeted Regulation Of S6K1 In The Proliferation,Metastasis And Invasion Of Cervical Cancer Cells

Recently,the pass away of women on count of tumor is mostly put down to CC.In spite of it attain the good therapy in clinical,and the death due to CC is lower than before in recent years.But for the refractory CC and the last period patients,it shows low cure rate,so the CC is recurrence easily.It puts a serious threat to women.MiRNA is endogenous.It is exits in single-copy or multi-copy.It can regulate the one-third genes of human.a variety of short-stranded RNAs have been found to regulate the behavior of CC cells.So,doing more test of miRNA is important for discover the new cure methods of CC.A lot of tests indicated that mir-195 can affect the cells of tumor.It is linked with the cellulate behavior of tumor.S6 Ks is the downstream protein of m TOR.And m TOR can phosphorylation S6K1 and 4E-BP1,then regulate the behavior of cells.As we can see,the absent of S6K1 and S6K2 s caused by some reasons could lead to the cell programmed death.We explored the targeted relationship between S6K1 and mir-195,and this targeted relationship could act on CC cells,and provide the basic for clinic.We choose 20 CC patents,get their CC texture and normal texture.Use PCR to detect the expression of mir-195.We choose Hela to primary foster.Choose the third dynasty transfect with the mir-195 mimics and inhibitor.Then,detected the expression of mir-195 in cells.We had verified the target relationship between mir-195 and S6K1 already.CCK8 detected the vitality of cells.TUNEL counted the number of programmed death of cells.Scratch detected the rate of cell migration.Transwell detected the penetrating power of cells.FCM measured the rate of G1 phase cells.The results indicated that the expression of mir-195 in CC texture is fewer than normal.The expression of mir-195 in mimics group is more than NC.And the expression of mir-195 in inhibitor is less than NC.We predict a target site between mir-195 and S6K1,and we verify it already by Dual-Luciferase.After boosted the expression of mir-195,the content of S6K1 has decreased.After reduced the expression of mir-195,the content of S6K1 has increased.CCK8 indicated that when the expression of mir-195 was enhanced,the cell viability of cells was decreased.It is on the contrary when the expression of mir-195 was decreased.TUNEL and FCM indicated that the number of programmed cell death was increased in mimics group and the programmed cell death was decreased in inhibitor group.FCM indicated that 70 percent of cells were in G1 phase in mimics group.And just less than half cells were in G1 phase in inhibitor group.Scratch test indicated that the cells migration to the middle was faster in inhibitor group than mimics group.Transwell indicated that the invasion power of cells in mimics group was lower than inhibitor group.In summary,during the pathological development of cervical cancer,miR-195 exhibits tumor suppressor,S6K1 exhibits cancer-promoting characteristics.And S6K1 could be the target site of mir-195 to acted on the biological behaviors of CC cells.Boosted the expression of mir-195 could reduce the biological activity of CC cells.This gives some enlightenment to the clinical.