The Effect Of Interaction Between Nanostructured Substrate And Cell On Cell Behavior And Capture

Malignant tumors,or cancers,are still diseases that are difficult to cure at present.The immortal proliferation and strong metastatic characteristics of malignant tumors are the main causes of their fatalities,which seriously endanger human life and health.Circulating tumor cells(CTCs)are one of the few new tumor molecular markers that have been researched and applied in the past ten years and can be used for cancer detection,diagnosis and prognosis monitoring.However,the clinical application of antibody-based separation technology is limited due to the heterogeneity and difference of target antigen expression in different cells.In recent years,with the development of nanotechnology and the further improvement of tissue engineering,many researches on nano-materials have been gradually applied to many fields such as biomedicine and environmental science.Existing studies have shown that the surface of the substrate grown on nanostructures with a specific aspect ratio is a promising tool for studying cell processes and designing novel devices to control cell behavior.The interaction between cells and nanostructured substrates can simulate the growth of tumors.In the microenvironment,there is a certain connection between the changes in the mechanical properties of the microenvironment and the various behaviors of the cells.Therefore,it is necessary to fully understand the structure of nanomaterials and the coupling relationship during cell growth of nanomaterials in order to make full use of their unique characteristics.In this thesis,two kinds of nano forest arrays with different structures were prepared by plasma polymerization.Taking HeLa cells as an example,the effects of nano forest arrays with different mechanical properties on the capture ability of cancer cells were studied.The experimental and finite element simulation data show that the two kinds of silicon nano forest arrays prepared in this study can have different mechanical responses to the bending properties of cells,So as to produce different mechanical stimulation;The results showed that silicon nanoforest array changed HeLa cell adhesion morphology and migration behavior.NCW and nfcw substrates increased cell capture efficiency by 2-3 times and reduced cell migration efficiency by 20-30%;The results showed that the expression of vinculin in NCW was 1.7-2 times of that in other groups.Therefore,the high expression of vinculin in NCW was the main reason for NCW to enhance cell capture ability and reduce cell migration.In summary,nanostructures can be used as a promising method to isolate CTCs,regardless of the expression of surface markers or the size of CTCs.The silicon nanoarrays with a specific nanoscale morphology prepared by us can enhance the interaction with cells,improve cell capture efficiency without using capture antibodies,and provide good biocompatibility at the same time.Ensuring the activity of the captured cells is conducive to the subsequent detection and characterization of the captured cells.This research has selected an optimized nano-morphology to provide useful information for further improving cell capture efficiency,which also provides some help for the clinical application of nano-substrates and cancer treatment.