Study On The Difference Of Immunoglobulin And Complement In HBV-related Liver Diseases And Its Correlation With The Clinicopathological Features Of Hepatocellular Carcinoma

Objective: To investigate the variability of immunoglobulin and complement in patients with HBV-related liver disease and also to analyze their correlation with clinicopathological features of hepatocellular carcinoma,in order to provide a reference for assessing patients’ conditions and guiding clinical treatment.Methods:(1)A retrospective analysis of the clinical data of 310 patients with HBVrelated liver disease who were hospitalized for the first time in the Affiliated Hospital of Youjiang Medical University for Nationalities from January 1,2016 to September 30,2019,of which 111 cases were in the chronic hepatitis B group and 98 cases were in the hepatitis B cirrhosis group,101 cases of hepatocellular carcinoma group.Collect the immunoglobulin and complement results of the above groups of cases,and compare and analyze the differences between immunoglobulin and complement in patients with different HBV-related liver diseases.(2)Retrospective analysis of clinical data of 106 patients with primary hepatocellular carcinoma who were hospitalized in surgery and underwent hepatectomy during the same period.Collect immunoglobulin and complement results,clinical conditions,and pathological characteristics of all patients,group them according to clinical conditions and pathological characteristics,and analyze the correlation between immunoglobulin and complement and clinicopathological characteristics of hepatocellular carcinoma.Results:(1)There were statistically significant differences in serum Ig A,Ig G,Ig M,C3,and C4 levels in the chronic hepatitis B group,hepatitis B cirrhosis group,and hepatocellular carcinoma group(P<0.01).The levels of Ig A,Ig G,and Ig M in the hepatitis B cirrhosis group were higher than those in the chronic hepatitis B group and hepatocellular carcinoma group(P<0.01).However,there was no significant difference in the levels of Ig A,Ig G,and Ig M between the chronic hepatitis B group and the hepatocellular carcinoma group(P>0.05).The contents of C3 and C4 in the hepatocellular carcinoma group were higher than those in the chronic hepatitis B group and hepatitis B cirrhosis group(P<0.01),while the contents of C3 and C4 in the liver cirrhosis group were lower than those in the chronic hepatitis B group(P<0.01).(2)There was no statistically significant difference in serum Ig A,Ig G,Ig M,C3,C4 levels in different gender groups and in different AFP groups(P>0.01).In the comparison between the HBs Ag positive group and the negative group,the level of complement C3 in the positive group was lower than that in the negative group,the difference was statistically significant(P<0.01),and the other indicators were not statistically significant(P>0.05).Ig A,Ig G levels and age were positively correlated(P<0.05),Ig M,C3,C4 and age were not correlated(P>0.05).(3)The differences in the levels of Ig A,Ig G,Ig M,C3,and C4 in the combined portal carcinoma embolism and no portal carcinoma embolism groups were not statistically significant(P>0.05).The levels of complement C3 and C4 in the tumor>5cm group were higher than those in the tumor≤5cm group,the difference was statistically significant(P<0.01),and the other indicators were not statistically significant(P>0.05).The level of complement C3 in the group with cirrhosis was lower than that in the group without cirrhosis,and the difference was statistically significant(P<0.01),while the remaining indicators were not statistically significant for comparison(P>0.05).There was no correlation between Ig A,Ig G,Ig M and BCLC staging(P>0.05),positive correlation between complement C3 and C4 levels and BCLC staging(P<0.01),negative correlation between Ig G levels and degree of differentiation(P<0.05),positive correlation between complement C3 levels and degree of differentiation(P<0.05),and no correlation between Ig A,Ig M,C4 and degree of differentiation(P>0.05).Conclusion:(1)Immunoglobulin Ig A,Ig G,and Ig M levels in patients with HBV-related liver disease increased with the aggravation of liver lesions;while complement C3 and C4 levels decreased with the aggravation of liver lesions and showed a tendency to increase in primary hepatocellular carcinoma,the specific mechanism of which may be related to the immunosuppression of hepatocellular carcinoma cells by complement.(2)There was no significant correlation between immunoglobulins and clinicopathological features of hepatocellular carcinoma,while there was a positive correlation between complement levels and tumor size,differentiation degree,and BCLC stage,which is of clinical significance for assessing anti-tumor immune function and disease in patients with hepatocellular carcinoma.