Inhibitory Effect Of Cinnamaldehyde On Osteosarcoma And Its Mechanism

Background: Osteosarcoma is a primary bone tumor associated with locally aggressive growth and early metastatic potential that typically occurs in children and adolescents.Chinese traditional medicine Cinnamomum cassia Presl has been shown to have significant tumor-killing effect,in which cinnamaldehyde(CA)is the main active ingredient.Purpose: To explore the anticancer effect of CA on the osteosarcoma cells and the possible molecular mechanism.Methods: Crystal violet assay,MTT assay and colony-forming assay were used to confirm the inhibitory role of CA in the proliferation of 143 B and MG63 osteosarcoma cells.Hoechst 33258 staining and flow cytometry were used to observe apoptosis of 143 B and MG63 osteosarcoma cells.The migration and invasion role of 143 B and MG63 osteosarcoma cells were evaluated using transwell assays and wound healing assays.Western blot was used to analyze the expression levels of proliferation,migration,invasion and apoptosis related proteins and the underlying molecular mechanisms.Results: According to crystal violet assay,MTT assay and colony-forming assay,CA significantly inhibited cell proliferation.Hoechst33258 staining and flow cytometry analysis showed that CA-induced apoptosis in a concentration-dependent manner.The results of flow cytometry analysis also showed that CA can block 143 B cells in G2/M phase,and CA can block MG63 cells in G0/G1 phase.In addition,transwell assays and wound healing assays showed that CA inhibited the migration and invasion of osteosarcoma cells.In vivo mouse models,CA inhibited the growth of osteosarcoma.The results of Western blot showed that CA could change the expression levels of proliferation,migration,invasion and apoptosis related proteins in osteosarcoma cells,and its potential mechanism might be to inhibit the transcriptional activities of Wnt/β-Catenin and PI3 K / Akt in osteosarcoma cells.Conclusion: CA may inhibit the proliferation,migration,invasion and promote apoptosis of osteosarcoma cells by inhibiting Wnt/β-catenin and PI3K/Akt signaling pathways.CA may be a potentially effective anti-tumor drug.