The Effect Of PTPRN Gene On The Proliferation,migration And Invasion Of Human Glioma Cells

Human brain glioma is the most common tumor of the central nervous system.The most malignant glioblastoma(GBM)accounts for 50% of all glioma cases.The recurrence rate is high and the patient’s prognosis is poor.The life span is extremely short.Epithelial-mesenchymal transition(EMT)not only gives stem cells the characteristics of tumor cells,but also promotes the proliferation,invasion and migration of tumor cells.Therefore,inhibiting the occurrence of EMT in tumor cells is one of the hotspots of clinical research.In this study,4 cases of human glioma cancer tissues and adjacent tissues were used for transcriptome sequencing,and the data were analyzed and mined to screen target genes closely related to the spread and metastasis of gliomas.Then at the cell,gene and protein level,the target gene is functionally verified.The results of the study are as follows:1.After an in-depth comprehensive analysis of the transcription data,the PTPRN gene was screened out as a target gene closely related to the spread of human glioma.This gene is significantly up-regulated in GBM tissues,and the expression level is different in different types of GBM.It is characterized by almost no expression in glioblastoma(neurons),whereas an increase in the expression of the other three types(e.g.classical,mesenchymal and neuronal front).2.The expression level of PTPRN in different areas of glioma tissue is significantly different.The expression of PTPRN in the core area of glioblastoma(CT)and the core area of angiogenesis(CThbv)is extremely low.The expression of peripheral necrotic pseudopallitic cell area(CTPan)is lower,the expression of infiltrating tumor tissue(IT)is increased,and the expression of tumor edge/front(LE)is the highest,which is significantly higher than the other four areas(p < 0.001).3.Validated at the cellular level,knocking down the PTPRN can significantly inhibit the proliferation,migration and invasion of glioma cells.4.The analysis of related genes in the signal regulation pathways that PTPRN may participate in found that most genes,such as CDH1,CDH2,TWIST1,SNAI1,SNAI2,MMP-2,MMP-9,and VIM are closely related to the occurrence of EMT.5.Western blot analysis showed that the expression levels of mesenchymal cell surface markers N-cadherin,metal matrix protease MMP-9 and repressor protein Snail1 decreased,and the expression levels of epithelial cell surface marker E-cadherin increased,(P <0.05),indicating that after knocking down PTPRN,the EMT level of glioma cells is significantly reduced,thereby,the spread of glioma cells also inhibited.The above research results have laid the necessary foundation for revealing the spreading mechanism of human glioma,improving the diagnostic criteria of glioma,and developing targeted drugs for the treatment of glioma.