Correlation Analysis Of The Expression Of IL-17A And T Lymphocyte Subsets In Patients With Advanced Non-small Cell Lung Cancer

Objective:To compare the differences in the expression of serum IL-17A and T lymphocyte subsets in healthy subjects and advanced NSCLC patients,and to explore the relationship between the expression levels of IL-17A and T lymphocyte subsets in advanced NSCLC patients and the clinicopathological characteristics of patients and their correlation analysis In order to understand whether IL-17A can be an indicator of the immune function status of advanced NSCLC patients.Methods:Strictly based on the inclusion and exclusion criteria,70 newly diagnosed patients with advanced NSCLC diagnosed in the Oncology Department of the Affiliated Hospital of Chengde Medical College from November 2018 to April 2020 were included in the experimental group,and they were admitted to the Affiliated Hospital of Chengde Medical College for physical examination during the same period.Seventy healthy subjects from the Department of Health were included in the control group.The serum levels of IL-17A in the two groups were determined by ELISA,and the levels of CD3~+,CD4~+,and CD8~+T lymphocyte subsets in peripheral blood were determined by flow cytometry,and CD4~+/CD8~+was calculated.For the ratio of T lymphocytes,two independent sample t-tests were used to compare the differences in the expression of IL-17A and T lymphocyte subsets in normal people and NSCLC patients.The correlation between IL-17A and CD3~+,CD4~+,CD8~+,CD4~+/CD8~+in NSCLC patients was analyzed by spearman correlation analysis,and the difference was statistically significant with P<0.05.Results:1.The expression level of IL-17A in the serum of advanced NSCLC patients was higher than that of healthy individuals,and the results were statistically significant(P<0.01).2.The absolute values of CD3+,CD4+and CD8+lymphocyte subsets in peripheral blood of patients with advanced NSCLC were lower than those of healthy subjects,while the ratio of CD4+/CD8+was higher than that of healthy controls.(P<0.05).3.There is no significant difference in the expression of IL-17A in serum of advanced NSCLC patients in different genders,ages,pathological types and clinical stages(P>0.05).4.There is no significant difference in the expression of the absolute value of CD3~+,CD4~+,CD8~+lymphatic subgroups and the CD4~+/CD8~+ratio in peripheral blood of patients with advanced NSCLC in different genders,ages,pathological types and stages(P>0.05).5.There was no correlation between serum IL-17A expression level and blood CD3~+,CD4~+,CD8~+,CD4~+/CD8~+expression level in advanced NSCLC patients(P>0.05).Conclusion:1.IL-17A is highly expressed in the serum of advanced NSCLC patients,and the absolute value of CD3~+,CD4~+and CD8~+lymphocyte subsets are low in the blood of NSCLC patients,and there is no obvious correlation with the clinicopathological characteristics of advanced NSCLC.The immune status of advanced NSCLC patients Obviously inhibited,preliminary discussion of the detection of the above indicators can provide a certain reference for the diagnosis of advanced NSCLC.2.The correlation between IL-17A in serum of advanced NSCLC patients and T lymphocyte subsets in blood is not obvious,and the sample size needs to be expanded for further investigation.