Pathological Features Of Solid Adenoid Cystic Carcinoma Of Lacrimal Gland And Expression Of PI3K/Akt Signaling Pathway Related Proteins

Objective:To investigate the pathological characteristics of solid type LGACC and the role of PI3K/ Akt signaling pathway related proteins in its occurrence and development.Methods:From January 2010 to January 2019,the lacrimal gland pathological tissue of 19 patients(19 eyes)with solid type LGACC admitted to the Department of Ophthalmology of the Third Medical Center of PLA General Hospital and Department of Ophthalmology of Chengde Medical College were collected as the experimental group,and 8 patients(8 eyes)who underwent orbital content removal for other diseases were collected as the control group.The histopathological characteristics of lacrimal gland were observed by Hematoxylin-eosin(HE)staining,and the expressions of PI3 K,Akt,GLUT-1 and c-Met in lacrimal gland were detected by immunohistochemical staining.Results:The results of HE staining showed that the normal lacrimal gland tissue was composed of acinus and ducts under light microscopy.The acinus was uniform in size and clear in structure.The epithelial cells of ducts were rich in cytoplasm,the cells were arranged orderly and lymphocytes were infiltrated.The pathological manifestations of lacrimal gland in experimental group was as follows: normal lacrimal gland tissue was destroyed,cancer cells gathered into solid masses of different sizes,and coagulation necrosis was found in the center of some cancer nests.The cancer cells were small in volume,disordered in arrangement,darker nuclear staining and less cytoplasm.The cancer cells were similar to basal cells.And mitotic figures were common in tissue of LGACC.The positive expressions of PI3 K,Akt,Glut-1 and c-Met in the experimental group were significantly higher than those in the normal control group,with statistical significance(P<0.05).Conclusion:Pathological changes occurred in the lacrimal gland of patients with solid LGACC,including clump-like accumulation of cancer cells,coagulative necrosis in the center of the cancer nest,and rare mitotic figures,which may be related to excessive cell proliferation due to abnormal expression of proteins related to the PI3K/Akt signaling pathway.