Effects Of MicroRNA-1291 On Proliferation、Migration And Invasion Of Human Pancreatic Cancer PATU8988T Cells

Objective:To preliminarily investigate the role of microRNA-1291 in the proliferation,migration and invasion of pancreatic cancer cells in vitro,and its significance in the invasion and metastasis of pancreatic cancer cells.Methods:MicroRNA-1291 minic NC,microRNA-1291 minic,microRNA-1291 inhibitor NC and microRNA-1291 inhibitor were transfected into pancreatic cancer cell lines by Lipo3000.RT-qPCR was used to verify the transfection effects of microRNA-1291 minic NC,microRNA-1291 minic,microRNA-1291 inhibitor NC and microRNA-1291 inhibitor.CCK-8 method was used to detect the effects on the proliferation of pancreatic cancer cells were transfected by microRNA-1291 minic NC,microRNA-1291 minic,microRNA-1291 inhibitor NC and microRNA-1291 inhibitor.Cell scratch test detected the influence of microRNA-1291 minic NC,microRNA-1291 minic,microRNA-1291 inhibitor NC and microRNA-1291 inhibitor on the migration ability of pancreatic cancer cells after transfection.Transwell assay detected the effects of microRNA-1291 minic NC,microRNA-1291 minic,microRNA-1291 inhibitor NC and microRNA-1291 inhibitor on the migration and invasion ability of pancreatic cancer cells after transfection.Results:1.Compared with the blank control group,the expression of microRNA-1291 was not significantly different in PATU8988T cells after transfection with microRNA-1291 minic NC(P>0.05),but microRNA-1291 was overexpressed in PATU8988T cells after transfection with microRNA-1291 minic(P<0.05).Compared with the blank control group,there was no significant difference in the expression of microRNA-1291 after transfection with PATU8988T cells with microRNA-1291 inhibitor NC(P>0.05),while the expression of microRNA-1291 was inhibited after transfection with PATU8988T cells with microRNA-1291 inhibitor(P<0.05).2.CCK-8 experiments: Compared with the blank control group,after transfection with microRNA-1291 minic NC for 24h,48h and 72h,there was no significant difference in OD value of the transfected cells(P>0.05),but after transfection with microRNA-1291 minic for 24h,48h and 72h,the OD value of the transfected cells decreased significantly(P<0.05),indicating that microRNA-1291 minic could significantly inhibit the proliferation of PATU8988T pancreatic cancer cells.Compared with the blank control group,after transfection with microRNA-1291 inhibitor NC for24h,48h and 72h,there was no significant difference in OD value of the transfected cells(P>0.05),while after transfection with microRNA-1291 inhibitor for 24h,48h and 72h,the OD value of the transfected cells increased significantly(P<0.05).This indicated that microRNA-1291 inhibitor could significantly promote the proliferation of pancreatic cancer PATU8988T cells.3.Cell scratch experiment: Compared with the blank control group,microRNA-1291 minic NC continuously transfected pancreatic cancer PATU8988T cells for 24h,the cell scratching experiment showed no significant difference in the mobility of the transfected cells(P>0.05).MicroRNA-1291 minic continuously transfected for 24h showed a significant decrease in the mobility of the transfected cells(P<0.05),indicating that microRNA-1291 minic could significantly inhibit the migration of pancreatic cancer PATU8988T cells.Compared with the blank control group,microRNA-1291 inhibitor NC continuously transfected pancreatic cancer PATU8988T cells for 24h,the cell scratches experiment showed no significant difference in the mobility of transfected cells(P>0.05),while 24h after continuous transfection with microRNA-1291 inhibitor,the mobility of transfected cells was significantly increased(P<0.05),indicating that microRNA-1291 inhibitor could significantly promote the migration of pancreatic cancer PATU8988T cells.4.Transwell migration assay: Compared with the blank control group,microRNA-1291 minic NC continued to transfect pancreatic cancer PATU8988T cells for 24h.Transwell assay showed no significant difference in the transfection ability of the transfected cells after 48h(P>0.05).MicroRNA-1291 minic continued to transfect for24h,showing that the transfection ability of the transfected cells was significantly decreased(P<0.05),indicating that microRNA-1291 minic could significantly inhibit the migration of pancreatic cancer PATU8988T cells.Compared with the blank control group,microRNA-1291 inhibitor NC continued to transfect pancreatic cancer PATU8988T cells for 24h.Transwell assay showed no significant difference in the migration ability of transfected cells after 48h(P>0.05),while microRNA-1291 inhibitor continued to transfect for 24h,showing significantly increased migration ability of transfected cells(P<0.05),indicating that microRNA-1291 inhibitor could significantly promote the migration of pancreatic cancer PATU8988T cells.5.Transwell invasion assay: Compared with the blank control group,microRNA-1291 minic NC continued to transfect pancreatic cancer PATU8988T cells for 24h,and Transwell assay showed no significant difference in the invasion ability of transfected cells after 48h(P>0.05).MicroRNA-1291 minic continued to transfect for24h,showing that the invasion ability of transfected cells was significantly decreased(P<0.05),indicating that microRNA-1291 minic could significantly inhibit the invasion of pancreatic cancer PATU8988T cells.Compared with the blank control group,microRNA-1291 inhibitor NC continued to transfect pancreatic cancer PATU8988T cells for 24h,Transwell assay showed no significant difference in the invasion ability of transfected cells after 48h(P>0.05),while microRNA-1291 inhibitor continued to transfect for 24h,showing significantly increased invasion ability of transfected cells(P<0.05),indicating that microRNA-1291 inhibitor could significantly promote the invasion of pancreatic cancer PATU8988T cells.Conclusion:The expression of microRNA-1291 was significantly decreased in pancreatic cancer cell lines,and microRNA-1291 had a significant impact on the proliferation,migration and invasion of pancreatic cancer cells,which may be closely related to the early metastasis and surrounding invasion of pancreatic cancer.Promoting the expression of microRNA-1291 can inhibit the proliferation,migration and invasion of pancreatic cancer cells,and microRNA-1291 may become a new therapeutic target and a new biochemical indicator for early diagnosis of pancreatic cancer.