The Expression Of SPNS2 In Hepatocellular Carcinoma And Its Relationship With Epithelial-mesenchyme Transition

Objective To explore the correlation between the expression of spinster homolog 2(SPNS2)in hepatocellular carcinoma(HCC)and its epithelial-mesenchymal transition(EMT).Methods From January 2018 to December 2019,Collect 10 fresh clinical specimens of HCC and 70 paraffin sections of HCC surgical specimens(including cancerous tissues and corresponding adjacent tissues)and their clinical related pathological data,and detect the two major markers of SPNS2 and EMT,N-cadherin,E-cadherin expression in cancer and adjacent tissues,statistical analysis of the correlation between SPNS2 and clinicopathological features and EMT.Results Immunohistochemistry,real time polymerase chain reaction(RT-PCR)and Western blot all showed that the expression of SPNS2 was higher than the corresponding adjacent tissues,and the difference was statistically significant(P<0.05).Among them,immunohistochemistry and Western blot showed that N-cadherin was in cancerous tissues.The expression level of E-cadherin was higher than that of adjacent tissues,while the expression of E-cadherin was lower than that of adjacent tissues(P<0.05).The analysis showed that the positive rate of SPNS2 in HCC was 60%,and it was positively correlated with N-cadherin and negatively correlated with E-cadherin.Combined with clinical data,it is also concluded that the expression of SPNS2 in HCC has no correlation with the patient’s age,gender,and degree of differentiation,but is closely related to tumor node metastasis(TNM)grade and vascular invasion.Conclusion SPNS2 is differentially expressed in HCC,and it may promote the occurrence of HCC by regulating EMT transcription factors.SPNS2 is closely related to TNM staging and microvascular invasion,and can be used for clinical prognosis assessment.