| Objective The ultrashort wave was used to intervene in the knee joint extension contraction model of rabbits,and the therapeutic effect of ultrashort wave therapy on articular capsule fibrosis and its mechanism were discussed.Methods 32 New Zealand rabbits were randomly divided into a blank control group(group O,no special treatment,and sacrificed after 12 weeks of free movement),a simple fixed group(group P,sacrificed after fixed for 8 weeks),and a natural recovery group(group N,After 8 weeks of fixation,the fixation was cancelled,and the spontaneous recovery was executed after 4 weeks.),The ultrashort wave treatment group(M group,the fixation was released after 8 weeks of fixation,and the ultrashort wave was sacrificed after 4 weeks).The fixation method was to fix the left knee joint of the rabbit in the fully extended position with cast-type plaster,ranging from the groin to the proximal toe.After sacrifice,the mobility of the knee joint after myotomy was measured using a joint mobility measuring instrument.Masson staining was used to detect collagen deposition in the joint capsule at the rear of the knee.RT-PCR and Western Blot were used to detect transforming growth factor at the joint capsule at the rear of the knee.m RNA and protein expression of β1(transforming growth factor-β1,TGF-β1)and connective tissue growth factor(CTGF).Results(1)Compared with the blank control group,the knee joint mobility of the fixed group was significantly reduced after myotomy(P <0.05),the collagen deposition in the joint capsule behind the knee joint,the m RNA and protein expression of TGF-β1,The m RNA and protein expression of CTGF was significantly increased(P <0.05).(2)Compared with the simple fixation group,there was no significant difference in knee mobility after myotomy in the natural recovery group(P> 0.05).Collagen deposition in the joint capsule behind the knee joint,m RNA and protein expression of TGF-β1 were significant.Decreased(P <0.05),m RNA expression of CTGF increased significantly(P <0.05),and protein expression of CTGF was not significantly different(P> 0.05);knee joint mobility increased significantly after myotomy in the ultrashort wave treatment group(P <0.05),collagen deposition,m RNA and protein expression of TGF-β1 in the joint capsule behind the knee joint were significantly reduced(P <0.05),CTGF m RNA expression was not significantly different(P> 0.05),and CTGF protein expression was significantly reduced(P <0.05).(3)Compared with the natural recovery group,the knee joint mobility of the ultrashort wave treatment group after myotomy was significantly increased(P <0.05),collagen deposition in the joint capsule behind the knee joint,m RNA and protein expression of TGF-β1,The m RNA and protein expression of CTGF decreased significantly(P <0.05).Conclusions(1)During fixation of the rabbit straight knee contracture model,joint dysfunction and fibrosis of the joint capsule at the rear of the knee occur.8 weeks of fixation can lead to stable joint contracture in the rabbit.As one of the important causes of joint-induced contractures,the fibrosis of the posterior joint capsule of the knee joint may be related to the increased expression of TGF-β1 and CTGF.(2)During the recovery of the rabbit straight knee contracture model,the joint dysfunction and the fibrosis of the joint capsule at the rear of the knee all recovered to varying degrees.(3)Compared with the natural recovery group,ultrashort wave may reduce the expression of TGF-β1 and CTGF in the joint capsule,reduce the fibrosis of the posterior joint capsule,and then improve the straight knee contracture.(4)Microthermal dose of ultrashort wave intervention can inhibit the progress of rabbit knee contracture.However,the optimal dose for treatment needs to be further explored. |
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