| Objective:To explore the effect of Atractylodes macrocephala broken wall decoction on the enteric neurotransmitter of STC mice and the SCF/c-kit signal pathway of ICC.Methods: The STC mouse model was established by subcutaneous injection of 2.5mg/(kg.d)morphine hydrochloride injection.Forty-eight Kunming mice with half male and female were randomly divided into normal group,model group,low,medium,and high dose groups(25,50,100 mg/kg)and mosapride group(1.5mg/kg).),8 per group.After each group was treated with different drugs for 1 week,the fecal properties of each group were observed and the intestinal ink transport distance was calculated after modeling and administration;the changes of mucosal inflammation were observed by HE staining of the colon of each group of mice;each group was measured by ELISA The contents of neurotransmitters SP,Ach,VIP,NO and 5-HT in the colon of mice in the group;WB was used to detect the expression of SCF and c-kit protein in the colon tissue of mice.Results: 1.General conditions of mice,each group of mice before modeling: active,uniform in coat color,and no abnormalities in fecal traits.After modeling,the mice in each group of the model were not active,moved lazily,their hair fell off,and the stools were separated hard balls or ball-shaped feces,indicating that the modeling was successful.After the administration,the fatigue and drowsiness symptoms of the mice in each group gradually improved,and the reaction was more sensitive than before the administration,and the sausage-like or ball-like smooth and soft stools increased.2.The results of mouse intestinal ink transport ratio showed that morphine injection significantly down-regulated the intestinal ink transport ratio of mice in each group of the model;after 1 week of administration treatment,it significantly increased the mosapride group and Baizhu decoction pieces The transport ratio of intestinal ink in mice in the middle-dose group and the high-dose group of Atractylodes macrocephala decoction.3.HE staining results showed that the colonic mucosa of the normal group was normal;the colonic mucosa of the model group showed obvious inflammatory changes mainly composed of lymphocytes;the mice in the low-dose Atractylodes macrocephala decoction pieces showed a small amount of inflammatory cells;There was no obvious mucosal inflammation in the colon of mice in the middle and high dose groups and mosapride group.4.ELISA results showed that compared with the normal group,the SP and Ach contents of the model group were significantly reduced(SP : 230.41±16.41 vs.146.69±15.59;Ach:577.68±39.35 vs.281.50±39.40).Compared with the model group,the SP content in the low-dose group of Atractylodes macrocephala,the middle-dose group and the high-dose group of Atractylodes macrocephala fragments were significantly increased(146.69±15.59 vs.188.25±4.60;146.69±15.59 vs.201.78±4.44;146.69±15.59 vs.307.51±16.94);Ach content in high-dose Atractylodes macrocephala and mosapride group were significantly increased(281.50±39.40 vs.400.93±12.21;281.50±39.40 vs.422.22±22.70).Compared with the high-dose group of Atractylodes macrocephala decoction pieces,the SP and Ach contents of the low-dose group of Atractylodes macrocephalus decoction pieces and the middle-dose group of Atractylodes macrocephalus decoction pieces were significantly reduced(SP : 307.51±16.94 vs.188.25±4.60;307.51±16.94 vs.201.78 ±4.44;Ach :400.93±12.21 vs.283.79±11.53;400.93±12.21 vs.327.81±14.77);SP content in the mosapride group was significantly reduced(307.51±16.94 vs.164.08±12.54).Compared with the normal group,the contents of VIP,NO and 5-HT in the model group were significantly increased(VIP:64.47±2.69 vs.87.74±2.93;NO:38.21±1.76 vs.42.78±1.69;5-HT:219.58±11.60 vs.276.08±7.97).Compared with the model group,the VIP content of the low-dose Atractylodes macrocephala decoction pieces,the medium-dose Atractylodes macrocephalus decoction pieces,the high-dose Atractylodes macrocephalus decoction pieces,and the mosapride group were significantly reduced(87.74±2.93 vs.75.58±2.08;87.74±2.93 vs.69.34±2.23;87.74±2.93 vs.66.37±1.93;87.74±2.93 vs.65.31±3.32);Atractylodes macrocephala medium dose group,Atractylodes macrocephala fragment high dose group and mosapride group NO and The content of 5-HT was significantly reduced(NO :42.78±1.69 vs.39.27±1.90;42.78±1.69 vs.37.43±1.30;42.78±1.69 vs.35.65±2.01;5-HT:276.08±7.97 vs.257.89±6.16;276.08±7.97 vs.226.79±10.49;276.08±7.97 vs.242.05±12.15).Compared with the high-dose Atractylodes macrocephala decoction pieces group,the contents of VIP,NO and 5-HT in the low-dose Atractylodes macrocephalus decoction pieces group were significantly increased(VIP: 66.37±1.93 vs.75.58±2.08;NO: 37.43±1.30 vs.42.73± 2.19;5-HT: 226.79±10.49 vs.269.87±10.91);VIP,5-HT in the middle-dose group of Atractylodes macrocephala and 5-HT in the mosapride group were significantly increased.5.Western blot results showed that compared with the model group,the c-kit protein content in the low-dose group of Atractylodes macrocephala was significantly increased(0.22±0.10 vs.0.37±0.08);the middle-dose group of Atractylodes macrocephala and the high-dose group of Atractylodes macrocephala both the SCF and c-kit protein content in the mosapride group and the mosapride group were significantly increased(SCF:0.60±0.19 vs.0.99±0.28;0.60±0.19 vs.1.17±0.34;0.60±0.19 vs.1.02±0.30;c-kit:0.22±0.10 vs.0.47±0.10;0.22±0.10 vs.0.58±0.13;0.22±0.10 vs.0.49±0.13).Conclusion: Atractylodes macrocephala broken wall decoction can increase the contents of SP,Ach and SCF/c-kit in the colon tissue of STC model mice,and reduce the contents of VIP,NO and 5-HT,thereby regulating the gastrointestinal function of mice and promoting the intestinal tract Peristalsis,improve constipation symptoms,and then achieve the effect of curing STC. |
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