Correlation Between Plasma Asprosin Level And Coronary Heart Disease

Objective Asprosin is a new type of fat factor secreted by white fat,which can rapidly induce glucose production,regulate the release of glucose from the liver and maintain normal blood glucose.Lack of it in the human body can lead to unique metabolic disorders,including partial disorders of lipid metabolism,accompanied by plasma insulin resistance.Hyperglycemia and hyperlipidemia are important risk factors for coronary heart disease.At present,studies have shown that plasma asprosin levels are related to diabetes mellitus(diabetes mellitus,DM)and lipid metabolism disorders,but the characteristics of plasma levels in patients with coronary heart disease(coronary heart disease,CHD)are not clear.In this study,the levels and changes of plasma asprosin in normal subjects,patients with coronary heart disease and patients with coronary heart disease complicated with type 2diabetes mellitus(type 2 diabetes mellitus,T2DM)were measured and compared,and the correlation between plasma asprosin level and coronary heart disease was analyzed.Methods A total of 150 outpatients and inpatients from the affiliated Hospital of Zunyi Medical University from September 2020 to February 2021 were divided into normal group(control group,n = 50)and coronary heart disease group(n = 50).The occurrence of acute myocardial infarction in coronary heart disease was divided into two groups: acute coronary syndrome group(acute coronary syndrome group)and chronic coronary syndrome group(chronic coronary syndrome group).Coronary heart disease with type 2diabetes group(nasty 50),acute myocardial infarction was divided into two groups:chronic coronary syndrome with type 2 diabetes(chronic coronary syndrome with type 2diabetes)and acute coronary syndrome with type 2 diabetes(acute coronary syndrome with type 2 diabetes).However,due to a variety of factors,the actual number of cases included in the study were 33,52(ACS:n=23;CCS:n=29)and 39(ACS combined with T2DMT2DM:n=24).All populations were matched in terms of age,sex,body mass index((BMI))and education level.(1)collect the routine examination and test results of all selected people.Including white blood cell count(WBC),neutrophil count(NEUT),lymphocyte count(LYMPH),total platelet count(PLT),serum uric acid(UA),creatinine level(Cr),total cholesterol(TC),triglyceride(TG),alanine aminotransferase(ALT),aspartate aminotransferase(AST),high density lipoprotein cholesterol(HDL-C),Low density lipoprotein cholesterol(LDL-C)and D-dimer(D-dimer),etc.(2)the severity of coronary artery disease was evaluated by Gensini score in patients with coronary heart disease and coronary heart disease complicated with type 2 diabetes mellitus,(3)fasting overnight blood samples were collected,the plasma Asprosin level was determined by ELISA method,and the differences of Asprosin among the three groups were compared and analyzed,and the plasma Asprosin concentration was used to distinguish the ROC curve between coronary heart disease(including coronary heart disease complicated with type 2 diabetes)and healthy people.Patients with coronary heart disease were divided into ACS group and CCS group,and then the plasma Asprosin concentration was used to distinguish the ROC curve of ACS and CCS.(4)the correlation between asprosin level and related indexes in each group was analyzed.(5)binary logistic regression was used to analyze the independent risk factors of coronary heart disease.Results(1)compared with the general data of the three groups,the age of normal group(56.94 ±11.78),coronary heart disease group(64.38 ±10.18)and coronary heart disease with type 2 diabetes mellitus group(64.13 ±10.60).There was significant difference between normal group and coronary heart disease and coronary heart disease complicated with type 2 diabetes group(P < 0.05).Smoking history in normal group [6(18.2%)VS27(81.8%)],coronary heart disease group [27(51.9%)VS25(48.1%)],coronary heart disease with type 2 diabetes group [25(64.1%)VS14(35.5%)].There were significant differences in smoking history between normal group and coronary heart disease group and coronary heart disease with type 2 diabetes group(P < 0.05).Hypertension history in normal group [10(30.3%)VS23(69.7%)],coronary heart disease group [32(61.5%)VS20(38.5%)],coronary heart disease with type 2 diabetes group [20(51.3%)VS19(48.7%)].There were significant differences in hypertension history between normal group and coronary heart disease group and coronary heart disease with type 2 diabetes group(P < 0.05).Plasma Asprosin in normal group was 50.72(40.27),Asprosin in coronary heart disease group was 99.69(69.61125.13),Asprosin in coronary heart disease with type 2 diabetes group was 101.53(89.39126.17).There were significant differences in plasma Asprosin between normal group and coronary heart disease and coronary heart disease complicated with type 2 diabetes mellitus group.The level of Asprosin in coronary heart disease with type 2 diabetes group was the highest.There were significant differences among normal group(5.98 ±1.74),coronary heart disease group(7.40 ±2.50)and coronary heart disease with type 2 diabetes group(8.69 ±2.90)(P < 0.05).The absolute value of neutrophils in normal group [3.68(2.86,5.27)],coronary heart disease group [4.33(3.61,5.66)],coronary heart disease with type 2 diabetes group[5.57(4.67,7.33)].There was significant difference in the absolute value of neutrophils between coronary heart disease complicated with type 2 diabetes group,normal group and coronary heart disease group(P < 0.05).The absolute value of neutrophils in coronary heart disease complicated with type 2 diabetes mellitus group was significantly different from that in normal group and coronary heart disease group(P < 0.05).The total cholesterol in normal group(4.54 ±0.85),coronary heart disease group(5.08 ±1.39)and coronary heart disease with type 2 diabetes mellitus group(4.13 ±0.95).There was significant difference in total cholesterol between coronary heart disease group and normal group and coronary heart disease complicated with type 2 diabetes group(P < 0.05).High density lipoprotein cholesterol in normal group(1.18 ±0.28),coronary heart disease group(1.19 ±0.28),coronary heart disease with type 2 diabetes group(0.95 ±0.24).There was significant difference in high density lipoprotein cholesterol between coronary heart disease complicated with type 2 diabetes group and normal group and coronary heart disease group(P < 0.05).Low density lipoprotein cholesterol in normal group(2.71 ±0.64),coronary heart disease group(3.09 ±0.85),coronary heart disease with type 2 diabetes group(2.55 ±0.67).There was significant difference in low density lipoprotein cholesterol between coronary heart disease group and normal group and coronary heart disease complicated with type 2 diabetes group(P < 0.05).(2)Asprosin distinguishes the ROC curve of coronary heart disease group(including coronary heart disease group with type 2 diabetes)and normal group,and the area under the curve is 0.92 × Asprosin to distinguish between ACS and CCS ROC curve,the area under the curve is 0.882.(3)the results of Asprosin correlation analysis in normal group showed linear correlation among: Cr(,UA(UA,0.412,0.017),ALT(0.493,0.004)and PLT(0.367,0.36,0.036).The results of Asprosin correlation analysis in patients with coronary heart disease showed linear correlation among: gensini(rbure0.970(p < 0.001),TG(rbure0.811,p <0.001),HDL-C(raster 0.786,p: < 0.001)and LDL-C(raster 0.662,p: < 0.001).The results of Asprosin correlation analysis in coronary heart disease complicated with type 2diabetes mellitus group showed linear correlation among: gensini(rbure0.850(p < 0.001),TG(rbure0.678)p < 0.001),HDL-C(rerex 0.883)p: < 0.001)and LDL-C(rbure0.856)(p:< 0.001).The results of correlation analysis showed that there was a linear correlation in CHD group with type 2 diabetes mellitus(P < 0.05).(4)univariate logistic regression analysis: smoking history,age,hypertension history,Asprosin,WBC,NEUT,D-dimer,TC,LDL-C were correlated with coronary heart disease.The independent risk factors of coronary heart disease were analyzed by multivariate logistic regression: smoking history(OR:3.297),hypertension history(OR:3.456),Asprosin(OR:1.083),WBC(OR:1.377),NEUT(OR:1.003),TC(OR:4.514),LDL-C(OR:0.307),age(OR:1.120).Conclusion(1)the level of plasma Asprosin was increased in patients with coronary heart disease and coronary heart disease complicated with type 2 diabetes mellitus,and the concentration was the highest in patients with coronary heart disease complicated with type 2 diabetes mellitus.(2)Asprosin is an independent risk factor for the occurrence of CHD,which can predict the occurrence of CHD to a certain extent.(3)the level of plasma Asprosin is of certain significance in the differential diagnosis of ACS and CCS.(4)in patients with CHD,whether with or without T2 DM,the Gensini score was positively correlated with Asprosin.The plasma concentration of Asprosin has a certain reference value for judging the severity of coronary artery in segmental patients.