Study On The Preparation And Properties Of HA/Collagen Composite Porous Bone Scaffold Materials

Bone defects caused by trauma,inflammation and infection are very common in clinical practice.Bone defects seriously affect the daily life of patients.The bone implant material can effectively achieve the purpose of treatment.In particular,bone-implanted materials have the advantages of overcoming the defects of autogenous and allogeneic bones,repairing large-area bone defects,and no pathogenicity.This study was based on electrospinning and impregnation techniques to prepare HA/collagen composite micro/nanofiber menbbranes that mimic the microscopic components of bones.It is planned to design three-dimensional porous through-structure bone graft scaffolds with compositional and structural biomimetic structures.Through surface morphological characterization(SEM),phase analysis(XRD),echanical properties testing,and biological performance testing(simulated mineralization,degradation and cell viability(CCK-8)),the electrospinning process parameters were investigated in turn.The effect of morphology,the effect of collection speed on the degree of ordering of composite fibers,and the effect of high-temperature sintering and impregnation on the morphology of composite fibers,and simulations of the mechanical properties of bone-implanted stent materials at different mineralization times,and finally studied The biological properties of the bone implanted scaffold material.The main results achieved are as follows:(1)When the concentration of PVP is 8wt%and the HA content is 7wt%,the diameter distribution of HA/PVP composite micro/nanofibers prepared by electrospinning is relatively uniform,between 500 and 600nm;the sintering temperature is 550℃,and the holding time is At 2h,calcined HA micro-nano ceramic fibers with uniform size were obtained.When the rotation speed was 2700r/min,the degree of fiber ordering reached 75%,and the directional alignment effect was best.(2)When the optimal concentration of collagen impregnation was 4wt%,immersed 2 times,and the concentration of glutaraldehyde was 25%,the prepared HA/collagen composite micro/nanofibers had good continuity and the fibers were homogeneous;when the simulated mineralization was 6h,Ca/P Up to 1.42,a large amount of HA was generated on the surface of the material,demonstrating that the HA/collagen composite micro/nano-fiber has good biocompatibility;the hydrophilic angle of the material is 59.43°,and the material is hydrophilic,which is favorable for cell adhesion and growth;The material was degraded in 10×PBS for different time.With the increase of time,the material quality gradually decreased,and the weight loss rate reached 16.13%after 6 hours of degradation,which provided sufficient space for the growth of cells.The PH value in the degradation process was basically stable at 7.10.(3)The porosity of the scaffold material obtained is between 79%and 88%,which meets the requirements for the porosity of the bone implant material.The mechanical properties of the material after simulated mineralization are improved,and Compressive strength increased from 4.27MPa to 6.54MPa.In the early stage of the human body,there is a certain degree of mechanical support;CCK-8 analysis results showed that the scaffold material was non-toxic(grade 0)to bone marrow-derived mesenchymal stem cells(MSCs),and MSCs were able to adhere and proliferate well.