| Background and ObjectivesThe self-defects of radiotherapy,chemotherapy and surgery seriously affect the curative effect of patients with colorectal carcinoma.With the in-depth study of traditional Chinese medicine,more and more traditional Chinese medicine with significant anti-tumor effect has been found and applied in clinic.Therefore,it has important clinical value to study the effect of traditional Chinese medicine on the growth of colorectal cancer cells.Studies have shown that Astragalus Polysaccharide(APS),an important active ingredient of Astragalus membranaceus,plays an important role in the treatment of liver carcinoma,breast carcinoma,lung carcinoma and other malignant tumor.So far,the effects of APS on the growth of colorectal carcinoma LOVO cells and its related mechanism have not been reported.In this study,colorectal carcinoma LOVO cells were used as the research object.The aim of this study was to investigate the effect of APS on the growth of colorectal cancer cells,and to preliminarily clarify the value of APS in the clinical treatment of colorectal carcinoma.Methods1.The prepared APS aqueous solution was quantified by the standard curve of glucose standard substance2.The CCK-8 experiment was used to research the effect of APS on the growth rate and doubling time of colorectal carcinoma LOVO cells.3.The plate colony forming assay was used to investigate the effect of APS on the colony forming ability of colorectal carcinoma LOVO cells.4.The effects of APS on the migration and invasion of colorectal carcinoma LOVO cells were studied by transwell chamber migration assay and Matrigel gel invasion assay,respectively.5.Western blot assay was used to study the expression of cyclin A,cyclin B,cyclin E and p21 in colorectal carcinoma LOVO cells treated with APS.Results1.The results of standard curve of glucose standard substance showed that prepared APS aqueous solution was 58.26 mg/mL.2.The results of CCK-8 assay suggested that,compared with the growth rate of colorectal carcinoma LOVO cells without APS treatment,the growth rates of colorectal carcinoma LOVO cells treated with different concentrations of APS were significantly slowed(P<0.01),and the doubling times of colorectal carcinoma LOVO cells treated with different concentrations of APS were significantly prolonged(P<0.01).According to the experimental results,combined with the state of cells in the experiment.In the follow-up experiment of this study,the experimental group and the control group were colorectal carcinoma LOVO cells treated with 200μg/mL APS for 48 h and parental colorectal carcinoma LOVO cells,respectively.3.The results of plate colony forming assay showed that:the average colony forming number of colorectal carcinoma LOVO cell in experimental group was significantly lower than that of colorectal carcinoma LOVO cell in control group(P<0.01),and the volume of cell clone in experimental group was smaller than that of control group.4.The data from transwell chamber migration assay and Matrigel gel invasion assay showed that,compared with the cell number of colorectal carcinoma LOVO cell through polycarbonate membrane in control group,the cell number of colorectal carcinoma LOVO cell through polycarbonate membrane was significantly low in experimental group(P<0.01).5.Western blot results certificated that the expressions of cyclin B and cyclin E in colorectal carcinoma LOVO cells of experimental group were evidently lower than that in colorectal carcinoma LOVO cells of control group(P<0.01),and there was no significant difference in the expression of cyclin A between the two groups(P > 0.05),while the expression of p21 was markedly higher in colorectal carcinoma LOVO cells of experimental group than that in colorectal carcinoma LOVO cells of control group(P<0.01).Conclusions1.APS has a significant inhibitory effect for the growth,migration and invasion of colorectal carcinoma LOVO cells。2.The changes of cyclin B,cyclin E and p21 expression may be involved in the inhibitory effect of APS on the growth,migration and invasion of colorectal carcinoma LOVO cells... |
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