Expression Of UBE2M In Colorectal Tumors And Its Effect On HCT116 Cell Proliferation And Cell Cycle

Background:Colorectal cancer(CRC)is the most common tumor in the digestive tract,and its occurrence is a multi-stage,multi-step complex process.At present,significant progress has been made in the molecular mechanism,diagnosis and treatment of colorectal tumors,but the 5-year survival rate of colorectal tumors has not improved significantly.Many studies have shown that ubiquitin-conjugating enzyme E2 M is closely related to the occurrence and development of a variety of tumors,but the role of ubiquitin-conjugating enzyme E2 M in colorectal cancer has not been clearly reported.Therefore,proving its role in colorectal cancer will help the diagnosis and treatment of colorectal cancer.Aim:To explore the expression of UBE2 M in colorectal cancer tissues and colorectal tumor cells HCT116,and the effects of UBE2 M on the proliferation,cloning and cell cycle of HCT116 cells.Finally,we will find an effective diagnostic marker and therapeutic target for colorectal tumors.Methods:The GEO and TCGA databases were used to obtain the expression level of UBE2 M in colorectal tumors,the prognostic impact of patients,and the relationship with G12 D mutations.THPA online data obtains the immunohistochemical status of UBE2 M in normal colorectal tissues and tumor patients.After sg RNA was transfected into HCT116 cells,the transfection efficiency was verified by Western blot experiment.CCK-8 was used to detect the proliferation ability of HCT116 cells;plate cloning experiment was used to detect the cloning ability of HCT116 cells;flow cytometry was used to detect the cell cycle distribution of HCT116 cells.Resuits:The online visual bio-information analysis software TIMER found that the UBE2 M gene was highly expressed in a variety of tumors,and it was statistically significant.UALCAN online software found that UBE2 M is differentially expressed in colorectal tumors and normal colorectal tissues(p<0.001).Kaplan-Meier found that high expression of UBE2 M showed a worse survival cycle than low expression.Using R language to analyze the GEO database showed that the expression level of UBE2 M in colorectal cancer was significantly higher than that in normal colorectal tissues(P<0.001).In the overall survival analysis,it was found that the survival period of patients with high UBE2 M expression was significantly lower than that of patients with low expression.(P<0.05).In addition,UBE2 M was found to be highly expressed in colorectal tumors with Kras mutations.Further analysis revealed that the differences were more obvious in the G12 D mutation point.The THPA database found that UBE2 M showed a difference in antibody staining levels between normal human colorectal tissues and colorectal cancer tissues(P<0.001).After knocking down UBE2 M in HCT116 cells,using CCK-8 and plate cloning experiments,it was found that the knockdown significantly inhibited the proliferation and cloning ability of HCT116 cells.Analysis of the lost cell cycle found that after the UBE2 M gene was knocked down,the number of cells arrested in the G2/M phase increased significantly in HCT116 cells.Conclusion:1.UBE2 M is highly expressed in colorectal cancer tissues,and is correlated with the overall survival of patients;2.UBE2 M was highly expressed in patients with G12 D mutation in colorectal cancer tissue.3.After down-regulating UBE2 M,the proliferation and cloning ability of HCT116 cells are inhibited,and a large number of HCT116 cells stagnate in the G2/M phase.