Inhibition Of Ibuprofen Combined With Chlorhexidine On Enterococcus Faecalis In Vitro

Objective:Enterococcus faecalis is an opportunistic pathogen.The colonization of Enterococcus faecalis can be easily found in root canal therapy failure and in the periodontal pockets of patients with severe periodontal disease.Inflammatory granulation tissue and bone resorption occur in the damaged area under long-term chronic inflammatory stimulation.Chlorhexidine gluconate is a biguanidine chlorobenzene with a broad spectrum of antimicrobial activity,but does not significantly improve the bone damage caused.Ibuprofen is a kind of ring oxidase inhibitors,Cytokines can inhibit the production of prostaglandins area and thus to minimize damage to bone absorption,the experiment in vitro determination of ibuprofen,glucose acid chloride has set the minimum inhibition concentration of dung enterococcus.Explore the ibuprofen and chlorhexidine combined application of dung enterococcus in vitro have synergy or antagonism effect.Objective To provide guidance for the adjuvant treatment of enterococcus faecalis infection.Materials and Methods:1.The minimum inhibitory concentration(MIC)of chlorhexidine gluconate and ibuprofen against Enterococcus faecalis was determined by double dilution method and AGAR plate dilution method,and the antibacterial strength of ibuprofen solutions with different concentrations against Enterococcus faecalis was determined by AGAR plate dilution method.2.The combined effect of two drugs(chlorhexidine gluconate + ibuprofen)on Enterococcus faecalis was determined by AGAR plate dilution method.Determine the combined action mode: the plate colony number under the combined action of two drugs was significantly lower than that under the single action of two drugs,it was determined that both drugs had a synergistic effect.If there is no significant difference in the number of colonies,it is judged that there is no interaction between the two drugs.If the colony number of the plate was significantly higher than that of the two drugs acting separately,the two drugs were determined to have antagonistic effect.Results:1.The MIC of chlorhexidine gluconate was 31.25μg/ml;The MIC value of ibuprofen was 2.3mg/ml.With the increase of the concentration of ibuprofen,the antibacterial effect on Enterococcus faecalis was also enhanced.When the concentration was increased to 2.3 mg/ml,the bacteria were completely inhibitory.2.Two drugs(chlorhexidine gluconate and ibuprofen at concentrations of 15.625μg/ml and 2mg/ml,respectively)were combined on Enterococcus faecalis,and it was found that the bactericidal ability of the combined action was stronger than that of the two drugs used alone.The results showed that chlorhexidine gluconate and ibuprofen had synergistic bactericidal ability against Enterococcus faecalis.Conclusion:1.The MIC of chlorhexidine gluconate was 31.25μg/ml.With the increase of ibuprofen concentration,the inhibition of Enterococcus faecalis also increased.When the concentration was 2.3mg/ ml,it was completely bacteriostatic.2.Chlorhexidine gluconate combined with ibuprofen has synergistic bacteriostatic effect.