INHBA And COL11A1 Promote The Proliferation And Invasion Of Colorectal Cancer Cells

Objective:Colorectal cancer(CRC)is still a very harmful malignancy,ranking among the top three malignant tumors in morbidity and mortality.It is of great interest to investigate specific diagnostic and therapeutic tumor markers for colorectal cancer.This study focuses on the expression and biological functions of INHBA and COL11A1 in colorectal cancer,followed by the specific biological mechanisms by which they exert these functions and the interaction between these two proteins.Finally,to elucidate the broader implications of their joint action in colorectal cancer.Methods:1.Bioinformatics analysis combined with a western blot to analyze the expression of INHBA in colorectal cancer.2.Silencing the expression of INHBA protein by transfection si RNA and observing the biological functions of colorectal cancer cells,including changes in cell proliferation,invasion,and migration.3.Co-expression analysis was performed to screen out the protein with the most consistent expression trend with INHBA in colorectal cancer and then analyze this protein’s expression and its effect on colorectal cancer cells’ biological function.4.Verify the binding of INHBA with another protein by Co-IP as well as cell transfection technique,verify the upstream and downstream regulatory relationship between them,and finally verify the effect of these two proteins on PI3K/ AKT/m TOR pathway.Results:1.INHBA protein was highly expressed in colorectal cancer cells.2.Silencing INHBA protein expression reduced the proliferation,invasion,and migration ability of colorectal cancer cells.3.Gene co-expression analysis showed a significant positive correlation between the expression of INHBA and COL11A1.Moreover,COL11A1 was also significantly highly expressed in colorectal cancer,and the proliferation,invasion,and migration ability of colorectal cancer cells were found to be correspondingly reduced after silencing COL11A1.4.Co-IP results showed that INHBA and COL11A1 had a binding relationship,but post-transfection analysis revealed that these two proteins were not upstream or downstream-regulated.Besides,we found that the proliferation,invasion,and migration of colorectal cancer cells were weaker with simultaneous silencing of INHBA and COL11A1 than with single gene silencing,suggesting that these two proteins have synergistic effects in colorectal cancer cells,and their high expression jointly promotes the growth and metastasis of colorectal cancer cells.Conclusion:Both INHBA and COL11A1 are highly expressed in colorectal cancer cells,and they have a binding relationship but not an upstream/downstream regulatory relationship.Their high expression in colorectal cancer cells jointly promotes the proliferation,invasion,and migration of colorectal cancer by activating the PI3K/AKT/m TOR pathway.This provides a basis for specific diagnosis and development of targeted therapeutic agents for colorectal cancer,and these potential capabilities need to be validated by other molecular biology and clinical trials.