The Role Of Acetylation In Regulating The Functions Of Ribosome In Salmonella Enterica

Acetylation is an important protein post-translational modification and widely present in eukaryotes and prokaryotes.As a switch of protein structure and activity,acetylation can regulate a series of cellular physiological processes.Previous study showed that the ribosomal proteins of Salmonella enterica serovar Typhimurium had extensively acetylation mediated by acetyltransferase Pat and acetyl-phosphate(Ac P).Here,we found that Ac P was involved in ribosome assembly,Pat contributed to maintaining normal translation efficiency,and proper acetylation was crucial for bacterial translation fidelity,suggesting the important roles of acetylation in regulating ribosome function.Moreover,we found that acetylation could mediate bacterial susceptibility to antibiotics,especially the ribosome-targeting aminoglycoside antibiotics.These results indicated that acetylation played important roles in the regulation of ribosome function and bacterial adaptation to complex environments.