The Analysis Of Peripheral Blood T Regulatory Cells And Th1/Th2/Th17 Cytokines In Chronic Rhinosinusitis

Objective: Chronic rhinosinusitis(CRS)is divided into different phenotypes which have been reported based on cytokine profile and inflammatory cell patterns.The aim of this study was to investigate the peripheral CD4+CD25+CD127+ regulatory T(Treg)cells and the Th1/Th2/Th17 cytokines in the serum of eosinophilic chronic rhinosinusitis(ECRS)or non-ECRS patients.Methods: The peripheral blood of 80 patients with CRS(34 ECRS patients and 46 non-ECRS patients)and 34 healthy subjects(control group)was collected.Flow cytometry was performed to detect the Th1/Th2/Th17 cytokines and Tregs.Results: In CRS subjects,the levels of IL-2,IFN-γ,IL-4 significantly decreased(P < 0.05)while levels of TNF-α,IL-6,IL-17 increased(P <0.01)compared with controls.ECRS patients presented with significantly lower levels of IL-2 and IFN-γ(P < 0.05)and higher levels of TNF-α,IL-6,IL-10,IL-17(P < 0.05)whereas non-ECRS patients showed significantly lower levels of IL-2,IL-4(P < 0.05)and higher levels of IL-6,IL-17(P <0.05).ECRS presented significantly higher levels of IL-10 compared with non-ECRS(P < 0.05).The Treg count in ECRS group was significantly lower than that in non-ECRS group(P < 0.01).Conclusion: Our findings suggest that ECRS subjects exhibit a decreased level of peripheral Tregs compared with non-ECRS subjects.Both ECRS and non-ECRS patients demonstrate impaired Th1/Th2/Th17 responses.