Renal Protective Effect Of Astragaloside-Ⅱ On Diabetic Rats

Background Diabetic nephrology(DN)is one of the common diabetic micrangium complication of diabetes mellitus(DM).Podocyte plays a key role in the occurrence and development of DN.A large number of studies have shown that mitochondrial dysfunction and autophagy defects are closely related to renal damage in DN.Traditional Chinese medicine has some advantages in the prevention and treatment of DN because of its multi-target and overall regulation.Astragalus membranaceus is widely used in the treatment of DN.Astragaloside Ⅱ(AS-Ⅱ)is one of the main active components of Astragalus membranaceus.However,there are few studies on its protective effect and mechanism on DN.In this study,diabetic rats were established to explore the protective effect and mechanism of AS-Ⅱ on DN.ObjectivesTo study the renal protective effect and molecular mechanism of AS-Ⅱ on diabetic rats,so as to provide a new strategy for the prevention and treatment of DN.MethodsIn this study,STZ induced type 1 diabetic rats were established and treated with AS-Ⅱfor 9 weeks.To observe the pathological changes of kidney,ultrastructure of podocyte,urinary microalbumin/creatinine(ACR)and expression level of related proteins in rats,so as to clarify the protective effect and underlying mechanism of AS-Ⅱ on podocyte injury in DN.ResultsAS-Ⅱ reduced urinary protein,improved pathological changes of renal,alleviated podocyte injury,ameliorated mitochondrial injury,restored autophagy level,as well as regulated Nrf2/ PINK1 pathway,had protective effect on DN renal injury.ConclusionAS-Ⅱ ameliorated podocyte injury and had protective effect on kidney in STZ-induced diabetic SD rats,and its mechanism may be related to the regulation of Nrf2 / PINK1 pathway.