The Relationship Between Livin Expression And Epithelial-mesenchymal Transition In Oral Squamous Cell Carcinoma And Its Clinical Significance

Objective:To observe the expression of Livin protein and Epithelial-mesenchymal transition(EMT)related proteins E-cadherin and Vimentin in oral squamous cell carcinoma(OSCC),to further analyze the relationship between the three proteins and the clinicopathological characteristics of OSCC,and to explore the correlation of the three proteins,in order to provide clinical data for the study of the possible molecular mechanism of the occurrence and development of OSCC.Methods:Immunohistochemical Elivision method was used to detect the expression of Livin,E-cadherin and Vimentin in 56 OSCC tissues(experimental group)and 56 corresponding adjacent normal tissues(control group),and SPSS 20.0 statistical software was used to analyze the final data.Results:1.Livin protein was mainly expressed in cytoplasm and/or nucleus,and the positive expression rate of Livin in OSCC tissues was 67.9%,which was significantly higher than that of 16.1% in adjacent normal tissues(P<0.05).The expression of Livin was significantly correlated with lymph node metastasis and TNM staging of OSCC(P < 0.05),but it had no correlation with gender,age,site of disease and degree of differentiation(P>0.05).2.E-cadherin is mainly expressed in the cell membrane,while Vimentin is mainly expressed in the cytoplasm.The positive expression rate of E-cadherin in OSCC(44.6%)was significantly lower than that in adjacent normal tissues(91.1%)(P < 0.05);Vimentin did the opposite(50.0% vs.8.9%).Both of them were significantly correlated with clinical stage,degree of differentiation and lymph node metastasis(P<0.05),and had no correlation with age,gender and site of occurrence(P>0.05).3.There was a negative correlation between the expression of Livin and E-cadherin in OSCC tissues(r=-0.536,P < 0.05);Livin was positively correlated with the expression of Vimentin(r=0.382,P=0.004).Conclusion:1.Livin protein was highly expressed in OSCC tissues,and its expression level increased with the clinical stage and occurrence of lymph node metastasis of OSCC.However,the low or no expression of Livin protein in adjacent normal tissues suggests that the overexpression of Livin may be closely related to the occurrence and development of OSCC.Therefore,deepening the understanding of Livin protein may contribute to the clinical diagnosis and treatment of OSCC.2.The expression of E-cadherin was low and the expression of Vimentin was high in OSCC tissues,and both of them were correlated with clinical stage,degree of differentiation,and lymph node metastasis,while the expression was contrary in adjacent normal tissues.The down-regulated expression of E-cadherin is accompanied by the up-regulated expression of Vimentin,suggesting that EMT may occur in the development of OSCC,which may be related to the invasion and metastasis of OSCC to some extent.3.In OSCC tissues,the expression of Livin was also correlated with the expression of E-cadherin and Vimentin,which further suggested that Livin may participate in the EMT process of OSCC and regulate the expression of E-cadherin and Vimentin proteins.These suggested that Livin may become a new tumor marker and therapeutic target.