Effect Of Fecal Microbiota Transplantation On Intestinal Flora And IL-23 And IL-17 Levels In Hepatocarcinoma Mice

Objective:(1)To observe the effect of human hepatocellular carcinoma(HCC)fecal microbiota transplantation in the intestinal setting of mice by establishing animal models of HCC.(2)To observe the effects of fecal microbiota transplantation from healthy people and patients with hepatocellular carcinoma on the levels of IL-23 and IL-17 in hepatocarcinoma mice induced by Diethylnitrosamine(DEN).Methods:The 48 C57BL/6J male mice were divided into 6 groups: Group A0: 6 blank control group;group A1: 6 healthy human fecal microbiota transplanted mice as control group;group A2: 6 mice were transplanted with fecal microbiota from patients with HCC.B0 group: 10 mice in DEN induced HCC model group;group B1: 10 hepatocarcinoma mice induced by DEN transplanted by fecal microbiota from healthy people;group B2: 10 hepatocarcinoma mice induced by DEN were transplanted with fecal microbiota from patients with HCC.The hepatocarcinoma mice models were constructed by intraperitoneal injection of DEN,and the feces of healthy donors were extracted to make a diluent to gavage the mice in groups A1 and B1;the diluent of fecal microbiota from patients with HCC was gavage to the mice in groups A2 and B2.After gavage transplantation 14 days,6 groups of mice feces were collected,and the 6 groups of mice feces were sequenced using 16 S rDNA sequencing(16S rDNA sequencing)technology.The histopathology of liver and ileum in 6 groups of mice was observed by HE staining.ELISA method to detect the levels of IL-17 and IL-23 in serum and liver tissues of 6 groups of mice.Results:The 16 S rDNA fecal microbiota sequencing analysis showed that there were significant differences in species diversity between groups B0,B1,and B2(p<0.05).The intestinal flora of group B1 was more abundant than groups B0 and B2(p<0.05);Comparing the diversity index Chao1 between groups A2 and B2,the abundance of the A2 group is greater than that of the B2 group(p<0.05);the differences in the samples between the groups are differences in enterobacteria at the level of phylum,class,and order.2.Compared with the B2 group,the expression of IL-17 in the liver tissue of the A2 group increased(p<0.05);compared with the A2 group,the expression of IL-23 in the liver tissue of the B2 group decreased(p<0.05).3.The pathological tissues of mouse liver and ileum showed that there was no significant difference between A0,A1,and A2 groups,and there was no significant difference between B0,B1,and B2 groups in mice by DEN induced.Conclusion:1.The transplantation of fecal microbiota in healthy people and patients with HCC can affect the species distribution of intestinal flora in hepatocarcinoma mice and change the diversity of intestinal flora in mice.2.Fecal microbiota transplantation in patients with HCC can up-regulate the expression of IL-23 in liver tissues of mice;Fecal bacterial transplantation in healthy people and patients with HCC has no effect on the expression of liver tissue and serum IL-17 and IL-23 in hepatocarcinoma mice.