Transcriptome Analysis Of Mice Melanoma Cells Under Direct Current Electric Field

The mechanism of tumor metastasis remains a big challenge in the clinical treatment of cancer.Directed cell migration is closely related to tumor metastasis.The electrical signal may play an important role in regulation of tumor metastasis.While its molecular mechanism underlie is not clearly understood.In order to explore how the electric field regulates mice melanoma cells in terms of gene expression and signaling pathways,The RNA-Seq of B16-F10 cells were analyzed with electric field application.Under the electric field strength of 300 m V/mm,the mouse melanoma tumor cell B16-F10 showed obvious catholicity.Under 300 m V/mm electric field treatment,about 3000 up-regulated genes and 2613 expression down-regulated genes were identified.Some up-regulated genes are positively correlated with cell migration,such as Serpine1,Ctgf,Fosb,Fos,Fosl2,Junb,Id1,Id2,Id3,Adm,Cyr61,Thbs1,Egr1,these genes are up-regulated obviously,which indicated electric field play a role in promoting tumor cells migration.In the differential gene KEGG enrichment results,five signaling pathways involved in cell motility were changed significantly including cytoskeletal actin regulation,MAPK pathway,Focal adhesion pathway,and m TOR pathway,which showed the effect of electric field to promote cell motility.The genes that promote tumorigenesis after application of an electric field are also up-regulated,and several signal pathways related to tumors change significantly.Furthermore,Some other signal pathway such as the glycolysis and gluconeogenesis pathways,Alzheimer’s disease etc.were changed obviously,which showed the complicated biological effects of the electric fields.The Western Blot results showed that the phosphorylation levels of P70S6 K and FAK proteins increased significantly after treatment with 300 m V/mm DC.The activation of these proteins can also promote the cell migration as well.