Clinical Observation Of Jia Wei Jin Kui Shen Si Tang On The Proliferation And Apoptosis Of Multiple Myeloma Cells And Combined With Western Medicine Treatment In The Treatment Of Multiple Myeloma With Spleen-kidney Yang Deficiency Type

Objective:1.To explore the effect of Chinese medicine Jia Wei Jin Qui Shen Qi Tang(Jia Wei Jin Gui Shen Qi Tang,SQT)on the proliferation and apoptosis of myeloma RPMI8226 cells(RPMI8226).2.Observe the safe and effective effect of Chinese medicine Jiawei Jingui Shenqi Tang combined with Western medicine in the treatment of multiple myeloma(MM)of spleen-kidney yang deficiency type,as an adjuvant drug for the treatment of spleenkidney yang deficiency type multiple myeloma(MM)provides a certain reference basis and ideas.Methods:1.Experimental researchTake the RPMI8226 cells in the logarithmic growth phase according to the IC50 to divide the RPMI8226 cells grown in the logarithmic phase into: blank control group(only culture medium,no cells),negative control group(with cell suspension,no drugs)and SQT experimental group(10,20,30 mg/ml),cultured for 24,48,and 72 hours,respectively,the inhibition rate of SQT on the growth of RPMI8226 cells at each time point was measured by the CCK8 method;according to the growth inhibition rate,the logarithmic phase growth RPMI8226 cells were divided into a blank control group And SQT(10,20,30mg/ml)experimental group,after 24h,48h culture,flow cytometry was used to detect the changes of the concentration of each group’s apoptosis rate with time.2.Clinical researchFrom December 2019 to January 2021,40 multiple myeloma patients of spleen-kidney yang deficiency type who were hospitalized in the Department of Hematology at the Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine were collected.According to the principle of randomization,20 patients in the western medicine treatment + traditional Chinese medicine group were treated with western medicine treatment + Chinese medicine Jiawei Jingui Shenqi Tang;20 patients in the western medicine treatment group were treated with pure western medicine treatment.After 4 courses of regular treatment,clinical efficacy,TCM syndrome efficacy,bone pain score,quality of life score,adverse reaction evaluation and secondary clinical indicators(creatinine,hemoglobin,myeloma cells)in two groups of chemotherapy before and after chemotherapy were compared.Results:1.A certain concentration of Jia Wei Jin Kui Shen Qi Tang can inhibit the prolif eration of RPMI8226 cells and induce their apoptosis in vitro.2.Jia Wei Jin Kui Shen Qi Tang combined with western medicine in the treatment of MM patients with spleen-kidney yang deficiency type,compared with the western medicine treatment group,there are statistical differences in the TCM syndrome curative effect scores and bone pain scores(P<0.05),which can better improve the spleen and kidney Yang deficiency Syndrome scores and bone pain symptoms,but there is no significant difference between the two in terms of clinical efficacy,adverse reactions,and KPS scores of quality of life.3.Jia Wei Jin Kui Shen Qi Tang combined with western medicine in the treatment of MM patients with spleen-kidney yang deficiency type,compared with western medicine treatment group,can improve the HGB level to a certain extent,and the difference is statistically significant.But the effect on creatinine and bone marrow plasma cells was not statistically significant.Conclusion:Jia Wei Jin Kui Shen Qi Tang can inhibit the proliferation of RPMI8226 cells an dinduce its apoptosis in vitro;its combined treatment with western medicine can improve the syndrome of Chinese spleen-kidney yang deficiency and bone pain symptoms,and to a certain extent level,it can better improve HGB.It is safe and effective for clinical application.