Effect Of Electroacupuncture On Complement C1q And Microglial Phagocytosis In SAMP8 Mice Of AD Model

ObjectiveTo observe the effects of electroacupuncture on learning and memory ability and the expression of complement C1q、Iba-1 and CD68 in hippocampal CA1 region of SAMP8 mice,and to explore the effects of electroacupuncture on complement C1 q and microglial phagocytosis(Studies have confirmed that AD microglial cells can phagocyte synapses),so as to provide experimental basis for electroacupuncture to improve synaptic function.MethodsTwenty-four 7-month-old male SAMP8 mice were randomly divided into two groups(model group and electroacupuncture group)with 12 mice in each group.12 normal aging SAMR1 mice with similar genetic background and the same age were the control group.Mice in electroacupuncture group were fixed by self-made net at the age of 7 months,and Baihui(DU20),Dazhui(DU14)and Shenshu(BL23)points were selected for acupuncture intervention,in which Dazhui and Shenshu(alternating on both sides every other day)were connected with electroacupuncture treatment apparatus.Acupuncture was performed by the same person every day,and intervention was carried out from 9:00 am to 11:00 am every day.The electroacupuncture parameters were continuous wave with frequency of 2 Hz and current intensity of 1.5-2m A.The degree was that the hind limbs of mice tremble slightly without neighing.The needle was kept for 20min/ time,once/day,8 days as a course of treatment,and there were 3 courses of treatment with 2 days between each course of treatment.All mice were collected after Morris Water Maze test.Behavioral methods were used to evaluate the learning and memory ability of mice.Immunohistochemistry and Western blot were used to detect the expression levels of C1q、Iba-1 and CD68 in the hippocampus of mice.q PCR was used to detect the m RNA expressions of C1q、Iba-1 and CD68 in the hippocampus of mice.Results1.Results of the Morris Water Maze experiment: compared with the control group,the average escape latency of mice in the model group was significantly prolonged(P < 0.01),the residence time of the original platform quadrant was significantly shortened(P < 0.01),and the number of platform crossing was significantly reduced(P < 0.01).Compared with the model group,the average escape latency was decreased(P < 0.05),the original platform quadrant residence time was prolonged(P < 0.01),and the number of platform crossing was increased(P < 0.01)in the electroacupuncture group.2.Immunohistochemical results: compared with the control group,the average optical density values of Iba-1 and CD68 in the hippocampus of model group were significantly increased(P< 0.01);Compared with model group,the average optical density values of Iba-1and CD68 in hippocampus of electroacupuncture group were significantly decreased(P< 0.01).3.Western blot results: Compared with the control group,the relative expressions of C1q、Iba-1 and CD68 in the hippocampus of the model group were significantly increased(P<0.05);Compared with model group,the relative expression levels of C1q、Iba-1 and CD68 in hippocampus of electroacupuncture group were decreased(P< 0.05).4.q PCR results: compared with the control group,the m RNA relative expression levels of C1 q and CD68 in model group were significantly increased(P< 0.01);Compared with model group,the m RNA relative expression levels of C1 q and CD68 in electroacupuncture group were decreased(P< 0.01).Compared with the control group,the m RNA relative expression of Iba-1in model group was increased(P< 0.05).Compared with the model group,the m RNA relative expression of Iba-1 in the electroacupuncture group showed a decreasing trend,but the difference was not statistically significant.Conclusions1.Electroacupuncture can improve the learning and memory ability of SAMP8 mice.2.Electroacupuncture can reduce the expression levels of C1q、Iba-1 and CD68 in the hippocampus of SAMP8 mice.3.Electroacupuncture may improve the synaptic function of SAMP8 mice by inhibiting the phagocytosis of complement C1 q and microglia.