Effects Of Lithospermum Polysaccharide On The Proliferation,Apoptosis And HPV18 E6/E7 MRNA Expression Of Cervical Cancer Cell Line Hela

Background: Cervical cancer is a common gynecological malignant tumor,the study found that high-risk HPV continued infection,especially 16,18 continued infection and cervical precancerous lesions and the occurrence of cervical cancer closely related.After HPV invasion,through DNA integration and expression,it induces the binding of E6/E7 oncogenes with tumor suppressor genes p53 and p Rb,leading to the inactivation of tumor suppressor proteins,disrupting the normal growth cycle of cells,promoting the immoralization of cells and eventually leading to cancer transformation.Currently,the treatment of HPV infection is limited,and new anti-HPV drugs are urgently needed.As a water-soluble component of traditional Chinese medicine,Lithospermum polysaccharide have been shown to be resistant to low-risk human papillomavirus and herpetic virus,and to a variety of tumors such as mouse cervical cancer U14 solid tumor,5180 ascites tumor and human gastric cancer cell(sgc-7901).However,little research has been done on the effect of lithospermum polysaccharide on Hela cells of human cervical cancer and its possible mechanism.Objective: The study aims to study the lithospermum polysaccharide effects on cervical cancer Hela cells,and to detect cell proliferation,cycle,apoptosis,and expression of HPV18E6 and E7 mRNA after lithospermum polysaccharide treatment,not only provides new evidence for antitumor,and also provides new options for the treatment of HPV infection and the prevention of cervical cancer.Methods: Hela cell lines(including high-risk HPV18)were cultured in vitro,and were divided into experimental group and control group.CCK8 assay was used to detect the inhibitory effect of different concentrations of lithospermum polysaccharide on cell growth,and annexinv-fitc/PI double staining flow cytometry was used to detect its effect on cell apoptosis.Real-time fluorescence quantitative PCR was used to detect the expression of HPV18E6/E7 m RNA.Results: Lithospermum polysaccharide inhibited the growth of cervical cancer Hela cells in a time-and dose-dependent manner.Hela cell apoptosis was induced by polysaccharide in a concentration-dependent manner.The relative expression of HPV E6/E7 m RNA detected by PCR showed a trend of decline with the increase of drug concentration after 24 h treatment of Hela cells with nori polysaccharide..Conclusion: Lithospermum polysaccharide can inhibit the proliferation of human cervical cancer Hela cells,induce the apoptosis of Hela cells,and inhibit the expression of HPV18 E6/E7 mRNA in vitro.