Investigation Of SiRNA Targeted To HuR In Suppressing Proliferation And Inducing Apoptosis In A Nude Mouse Model Of Human Laryngeal Squamous Cell Carcinoma

ObjectiveTo explore the effect of small interference RNA targeting HuR gene on the proliferation and apoptosis of LSCC and its possible mechanism on the growth of LSCC,the animal model of transplanted tumor of human laryngeal squamous cell carcinoma in nude mice was established.Methods1.Tumors were established in nude mice by transplantation of the LSCC AMC-HN-8 cell line;2.Design experimental group and different control groups,take solid tumor tissue and weigh it,and compare the tumor inhibition rate between each group(%);3.Ki-67 and Caspase-3 protein expression in tumor cells were analyzed by immunohistochemistry.Routine pathological and electron microscopic examinations were used to determine tumor apoptosis and proliferation.4.RT-PCR was used to detect the expression level of HuR,COX-2 and Survivin m RNA in each group of tumor tissues.5.Western blot was used to detect the expression level of HuR,COX-2 and Survivin proteins in tumor tissues in each group.Results1.In nude mice treated by intratumoral injection of high dose HuR-si RNA,tumor growth was significantly inhibited compared with control mice and those treated with low dose HuR-si RNA(p< 0.05).2.Routine pathological examination showed increased tumor tissue necrosis and apoptotic changes in treated mice,and immunohistochemistry confirmed increased expression of Caspase-3and reduced Ki-67 expression,compared with the control groups(p < 0.05).3.Compared with the low-dose HuR-si RNA group,the m RNA expression of HuR,COX-2,and Survivin in the tumors of the high-dose HuR-si RNA group were reduced.At the same time,the m RNA expression was significantly decreased in the treated group compared with the control group(p < 0.05).4.In the high dose HuR-si RNA group,HuR,COX-2 and survivin protein levels,which were positively correlated,were significantly reduced compared with the control and low dose HuRsi RNA group(p < 0.05).ConclusionsIn the LSCC nude mouse xenograft model,RNA interference technology was used to silence the expression of HuR gene,inhibit the growth of tumor cells,and promote apoptosis.Corresponding COX-2 and Survivin gene expression was significantly reduced,and the expression was positively correlated.The RNA interference technology targeting HuR gene has certain value in gene therapy for laryngeal cancer.HuR may play a role in inhibiting tumor growth through COX-2 and Survivin genes,and is an effective target for LSCC treatment.