Effects Of Hepatitis B Vaccination On Hepatitis B Surface Antigen In Neonates And Its Metabolism In Vivo

Part 1 Prevalence of hepatitis B among pregnant women in our hospital from 2017 to 2020Objective:To investigate the prevalence of hepatitis B surface antigen(HBsAg)among pregnant women hospitalized in Obstetrics and Gynecology Department of our hospital from 2017 to 2020.Methods:Data collection was conducted on the quantitative results of hepatitis B virus serum markers and HBV DNA on pregnant women in the Obstetrics and Gynecology Department,Affiliated Hospital of North Sichuan Medical College from July 2017 to July 2020.Results:1.A total of 7827 pregnant women were included in the study,of which 620 cases were HBsAg positive,the positive rate was 7.92%,and the positive rate of HBsAg was positively correlated with the age of pregnant women(P=0.003).2.Among 620 cases of HBsAg positive pregnant women,the "HBsAg,anti-HBe,and anti-HBc positive" mode was the main infection mode,accounting for 72.26%,followed by " HBsAg,HBeAg,and anti-HBc positive" mode,accounting for 20.00%,in addition,there were three rare modes.3.396 cases of HBsAg positive pregnant women were tested for HBV DNA at the same time,and 31.57%of them were positive for HBV DNA.There was significant difference in HBV DNA distribution between HBeAg positive and HBeAg negative groups(P<0.001).4.Among 7207 cases of HBsAg negative pregnant women,2539 cases were anti-HBs negative,accounting for 35.23%of the uninfected women,and 4668 cases were anti-HBs positive,accounting for 64.77%.Conclusion:The infection rate of hepatitis B in pregnant and parturient women in our hospital is 7.92%,so it is necessary to strengthen the screening of hepatitis B,improve the understanding of the mode of transmission of hepatitis B in maternal,so as to take appropriate measures of maternal and infant blocking as soon as possible,and be alert to the suggestive effect of the results of serum markers of hepatitis B in newborn.Part 2 Effects of hepatitis B vaccination on hepatitis B surface antigen in neonates and its metabolism in vivoObjective:To investigate the effect of recombinant hepatitis B vaccine on the quantitative results of hepatitis B surface antigen(HBsAg)in newborns,and the variation of hepatitis B vaccine concentration in neonates with time.Methods:1.Data collection was conducted on vaccination history of the hepatitis B vaccine,quantitative results of HBsAg and anti-HBs and other information on newborns in the Department of Neonatology,Affiliated Hospital of North Sichuan Medical College from July 2017 to July 2020.2.Plasma samples from healthy subjects were randomly selected and divided into anti-HBs negative group and anti-HBs positive group.After adding concentration gradient recombinant hepatitis B vaccine,quantitative detection of HBsAg was performed to verify the effect of hepatitis B vaccination on HBsAg.Results:1.A total of 1417 neonates were included in the study,of which 306(21.59%)were positive,with quantitative results ranging from 0.104 IU/mL to 0.339 IU/mL.The positive results of HBsAg were mainly detected within 8 days after inoculation.2.657 newborns whose mothers were HBsAg negative were divided into groups according to the anti-HBs concentration of their mothers.The positive rate of HBsAg in neonates was significantly negatively correlated with the anti-HBs concentration of their mothers(P<0.001).3.In vitro verification experiment showed that the positive rate of HBsAg was positively correlated with the concentration of hepatitis B vaccine;when the concentration of hepatitis B vaccine reached 10 ng/ml,the result of HBsAg was negatively correlated with the original concentration of plasma anti-HBs(P<0.05).Conclusion:Hepatitis B vaccination can affect the detection results of HBsAg in neonate plasma,and the effect could be eliminated with a delay in detection time.Moreover,anti-HBs to some extent offset the effect of vaccination on neonatal HBsAg results.