| Objective: The purpose of this study is to detect the concentration of serum AKR1B10 protein before and after operation in patients with newly diagnosed oral cancer(confirmed by pathological examination)and to compare the concentration of AKR1B10 protein in the serum of healthy people(no tumor and noticable hematological abnormalities were confirmed by physical examination).The clinicopathological information of patients with oral cancer is collected to analyze the correlation between the concentration of serum AKR1B10 protein and clinicopathological features.Methods: Randomly selected 150 patients with oral cancer diagnosed by pathological examination without antineoplastic therapy in Hunan Cancer Hospital from March 2020 to July 2020 as the observation group.Another 50 healthy subjects were randomly selected as the control group to collect clinicopathological information of patients with oral cancer,including age,sex,tissue type,clinical stage,pathological grade,living habits etc.The concentration of serum AKR1B10 protein was detected by magnetic particle chemiluminescence,and the difference of serum AKR1B10 protein concentration before and after operation of oral cancer and the difference of serum AKR1B10 protein concentration between oral cancer patients and healthy controls were compared.SPSS25.0 statistical software was used for statistical analysis,and the Wilcoxon rank-sum test and Mann-Whitney rank-sum test were used to compare the differences among groups.The efficacy of the serum AKR1B10 protein concentration test was analyzed by the Receiver operating characteristic curve(ROC)to find the cut-off value of serum AKR1B10 protein concentration in oral cancer diagnosis and to analyze the relationship between AKR1B10 protein and clinicopathological features of the patients was analyzed.Results:1.The level of serum AKR1B10 protein in patients with oral cancer before operation was significantly higher than that after operation and healthy control group.Subgroup analysis showed that the preoperative serum AKR1B10 level in patients with buccal carcinoma was significantly higher than that in the healthy control group,and the preoperative serum AKR1B10 protein concentration in the tongue cancer group was also higher than that in the healthy control group,but the difference was not statistically significant;the preoperative serum AKR1B10 protein concentration in gingival carcinoma and retromolar area cancer was similar to that in the healthy control group,which may affect the analysis results because of the small number of samples.2.Comparison of serum AKR1B10 protein concentration in different subgroups of oral cancer before and after operation: the concentration of serum AKR1B10 protein in tongue carcinoma,buccal carcinoma,gingival carcinoma and retromolar area carcinoma after operation was significantly lower than that before operation.3.The preoperative concentration of serum AKR1B10 protein in well differentiated oral cancer was higher than that in healthy controls,and the concentration of serum AKR1B10 protein in moderately differentiated and poorly differentiated oral carcinoma was also higher than that in healthy controls,but there was no significant difference between the two groups(P > 0.05).4.There was no significant correlation between preoperative serum AKR1B10 protein concentration and oral cancer patients age,sex,smoking,drinking and differentiation,but there was a positive correlation between betel nut chewing habit and serum AKR1B10 protein concentration in oral cancer patients,and the preoperative serum AKR1B10 protein expression level was correlated with tumor diameter,and the expression level in patients with oral cancer with tumor diameter ≤ 2cm was significantly higher than that in patients with oral cancer with diameter > 2cm.5.The best critical value of exploratory application of serum AKR1B10 protein level in the diagnosis and screening of oral cancer was276.675pg/ml and the AUC value was 0.626.Among them,the best critical value of AKR1B10 in the auxiliary diagnosis of oral and buccal cancer was 297.785 pg/ml,which was the highest(0.707).Conclusion:1.The increased concentration of serum AKR1B10 protein in oral carcinoma is significant for diagnosising oral cancer,and the best critical value is 276.675pg/ml.It is especially suitable for the early diagnosis of oral and buccal carcinoma,the AUC value is the highest(0.707),and the best critical value is 297.785pg/ml.2.There is a positive correlation between betel nut chewing bad habits and serum AKR1B10 protein concentration in oral cancer,while smoking,drinking,age,and sex are not significantly related to the oral cancer. |
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