Study On The Effect Of 18β-glycyrrhetinic Acid On The Proliferation Of PC-3 Cells And Its Mechanism

Objectives:To study the toxicological effects of 18β-glycyrrhetinic acid on PC-3 prostate malignant tumor cells cultured in vitro,Including the functional effects on its proliferation,migration,invasion,and apoptosis,as well as the effects on the expression levels of PI3K,p-PI3K,AKT,p-AKT,and bcl-2 proteins,Explore the effect of the drug on tumor cells in vitro,and evaluate its application prospects and possible mechanism of action for the treatment of prostate malignant tumors.Methods:PC-3 cells were cultured in vitro,and suitable cells were selected for random grouping,and detected by CCK-8 method at 0,5,10,20,40,80,160μmol/L absorbance under the concentration gradient explored the half inhibitory concentration(IC50),and then grouped according to the IC50 and started the experiment.Continue to use the CCK-8 method to detect the effect of 18β-glycyrrhetinic acid on the proliferation of PC-3 cells;use the cell scratch test and the Transwell chamber experiment to detect the effect of 18β-glycyrrhetic acid on the migration and invasion of PC-3 cells;Use Annexin and PI double labeling method to label cells to detect the effect of 18β-glycyrrhetinic acid on the apoptosis effect of PC-3 cells.At the same time,use Tunel staining method to detect the degree of apoptosis;in terms of mechanism exploration,Western Blot method is used to detect the effect of apoptosis.The protein expression of PI3K,p-PI3K,AKT,p-AKT,and bcl-2 after the intervention of 18β-glycyrrhetinic acid.The experimental results obtained are analyzed and processed using Graphypad prism,Image J,Flowjo,SPSS and other software.Results:The cells are subcultured in good growth condition,with regular morphology and adherent growth.The IC50 value measured by CCK-8 method is 19.30μmol/L.After 24h,48h,72h drug intervention,compared with the Blank and NC groups,the cell proliferation inhibition rate of the experimental group measured by the CCK-8 method was statistically significant,and there was a more obvious inhibitory effect(P<0.05)And it is related to concentration;The results of cell scratch experiment and Transwell chamber experiment showed that after 18β-glycyrrhetinic acid treatment,the cell migration and invasion ability of the experimental group was inhibited(P<0.05),which was related to the drug concentration;while flow cytometry and Tunel staining method The results showed that the apoptosis of PC-3 cells in the experimental group after drug intervention was more obvious than that in the control group(P<0.05),and it was concentration-dependent.The cell protein expression detected by Western Blot method was more inhibited in the experimental group than in the control group(P<0.05),and it was related to the concentration.Conclusion(s):1.In vitro,18β-glycyrrhetinic acid has a proliferation inhibitory effect on PC-3 cells and is concentration-dependent;2,18β-glycyrrhetic acid has a relatively obvious migration and invasion inhibitory effect on PC-3 cells and is related to drug concentration;3.18β-Glycyrrhetinic acid has the effect of promoting apoptosis on PC-3 cells and is concentration-dependent;4.18β-Glycyrrhetinic acid on PC-3 cells may have the same mechanism as PI3K,p-PI3K,AKT,p-AKT The protein expression of bcl-2 is inhibited,and the amount of expression is related to the concentration of intervention drugs.