Abstract Curcumin Attenuates γ-ray Radiation Damage In GSE-1 Cells By Up-regulating SIRT1

Objective:The purpose of this study was to explore the mechanism of curcumin against radiation damage of human gastric mucosal epithelial cells(GSE-1cells),and to clarify the mechanism of curcumin(Cur)attenuating γ-ray radiation injury in GSE-1 cells by up-regulating silencing information regulatory factor 1(SIRT1),so as to provide a new theoretical basis for the treatment of radiation-related gastric injury.Methods:GSE-1 cells were selected as test subjects.Combined with the previous research of the research group,the radiation dose was 4 Gy and the observation time was 24 hours after irradiation to carry out the following experiments.In this study,radiation protective agent amifostine(WR-2721)was used as positive drug control.1.Part one: experimental grouping: control group,Cur 5 μM group,Cur10 μM group,Cur 20 μM group.The cell viability was detected by MTT method to determine the effect of curcumin on the viability of GSE-1 cells.2.Part two: experimental grouping: control group,irradiation group,irradiation + Cur 5 μM group,irradiation + Cur 10 μM group,irradiation +Cur 20 μM group,irradiation + WR-2721 group.The cell viability was detected by MTT method to determine the effect of curcumin on the viability of GSE-1 cells damaged by γ-ray radiation.3.Part three: experimental grouping: control group,irradiation group,irradiation + Cur 5 μM group,irradiation + Cur 10 μM group,irradiation +Cur 20 μM group.Malondialdehyde(MDA)concentration was detected by MDA-TBA method,superoxide dismutase(SOD)activity was detected by WST-8 method,SIRT1 protein content was detected by Western blot in each proup,and the effects of curcumin on oxidative stress indexes of MDA and SOD in GSE-1 cells damaged by γ-ray and the expression of SIRT1 protein in cells were determined.4.Part four: experimental grouping: control group,Cur 20 μM group,irradiation group,irradiation + Cur 20 μM group,irradiation + Cur 20 μM +SIRT1 inhibitor(EX527)group,MDA concentration was detected by MDA-TBA method,SOD activity was detected by WST-8 method,SIRT1 protein content was detected by Western blot in each group.To determine the effect of SIRT1 inhibitor EX527 on the protective effect of curcumin on GSE-1 cells induced by γ-ray radiation.Results:1.In the concentration range of this experiment,there was no significant difference in the proliferation activity of GSE-1 cells treated with different concentrations of curcumin(P>0.05),which indicated that curcumin had no significant effect on the proliferation of GSE-1 cells.2.In the concentration range of this experiment,compared with the radiation group,the cell viability of GSE-1 cells injured by radiation increased in a concentration-dependent manner after being treated with different concentrations of curcumin,and the difference was statistically significant(P<0.05).Compared with WR-2721,the effect of curcumin was similar to that of WR-2721 when the concentration of curcumin was 20μM(P>0.05).3.In the concentration range of this experiment,compared with the radiation group,the concentration of MDA decreased and the activity of SOD increased in different concentrations of curcumin in radiation-injured GSE-1 cells(P<0.01),indicating that curcumin can improve the antioxidant stress ability of GSE-1 cells injured by γ-ray radiation.4.In the concentration range of this experiment,compared with the radiation group,the SIRT1 protein of radiation-injured GSE-1 cells increased in a concentration-dependent manner after being treated with different concentrations of curcumin(P<0.01),and the effect of 20 μM curcumin was the most obvious,indicating that curcumin can increase the expression of SIRT1 protein in γ-ray-damaged GSE-1 cells.5.After curcumin treated GSE-1 cells were treated with SIRT1 inhibitor EX527,the cell viability and SOD activity decreased,and the concentration of MDA was higher(P<0.01),indicating that the protective effect of curcumin on γ-ray radiation-damaged GSE-1 cells was weakened after the addition of EX527.Conclusion:Curcumin attenuates γ-ray radiation damage of GSE-1 cells by enhancing the ability of anti-oxidative stress damage of GSE-1 cells,and its mechanism is related to the up-regulation of SIRT1 protein.