| Objective: The quality control methods and standards of Psychotria serpens L.were preliminarily established by systematically studying on its source,character identification,microscopic identification,TLC identification,routine inspection,ethanol-soluble extract,determination of total flavonoids and chelidonin content,fingerprint,antibacterial activity and so on.And provide experimental basis for its further development and research.Methods: Through the base source identification,character and microscopic identification,physical and chemical identification methods to systematically qualitative study on pharmacognosy of Psychotria serpens L.The methods of determinations of moisture,total ash,acid-insoluble ash and ethanol extract were according as The Chinese Pharmacopoeia published in 2010.Study on the components of Psychotria serpens L.according to the chemical pretest method of traditional Chinese medicine.Used the orthogonal test to optimize the extraction process of total flavonoids from Psychotria serpens L.,by UV-Vis determined the contents of total flavonoids;with asperuloside as the reference substance,by HPLC determinating the asperuloside content of each batch Psychotria serpens L.from Guangxi,and preliminary researched the fingerprint and antibacterial activity of it.Results: 1.The original plant identification,morphological characteristics,microscopic identification and TLC identification have significant characteristics,which can be used as the basis for the identification of Psychotria serpens L.2.The moisture content in the Psychotria serpens L.between 5.31% and 11.20%,all bellowed 12.0%,so it must no more than13.00%;the total ash between 4.77% and 7.06%,and most of bellow7.0%,so it couldn’t be higher than 8.00%;the acid-insoluble ash between0.09% and 0.87%,but most bellow 0.7%,so it should not exceed 0.9%;the content of ethanol extracts between 23.88% and 35.30%,all above23.0%,so it shall not be less than 21.0%.3.It shows that flavonoids,anthraquinones,saponins,triterpenes,alkaloids,tannins,organic acids are included in Psychotria serpens L.4.The optimum extraction technology of total flavonoids in Psychotria serpens L.was as follows:0.5g of Psychotria serpens L.powder was precisely weighed,70% ethanol was used as extraction solvent,liquid material ratio1:100,the extraction time was 30 minutes;rutin showed a good linear relationship in the range of 0.002072 ～ 0.06216 mg/ml,the average recovery was 99.67%,and the total flavonoid content was 5.51%～9.97%,therefore,the proposed total flavonoid content should not be less than 3%.5.The optimum extraction process of Asperuloside was as follows:Precise weighing of Psychotria serpens L.powder 0.5g,using 70% methanol as extraction solvent,liquid material ratio 1:60,extraction time was30min;asperuloside showed a good linear relationship in the range of0.00623mg/ml～0.623mg/ml,the average recovery was 100.72%,and the content of Asperuloside in 15 batches of Psychotria serpens L.was between 0.0281% ～ 0.4679%,therefore,the proposed Asperuloside content is not less than 0.02%.6.The HPLC fingerprint and chromatographic conditions of Psychotria serpens L.were established preliminarily,screened 11 common characteristic peaks,rutin was used as the reference peak.The similarity of fingerprints of 15 batches of Psychotria serpens L.and control fingerprint was 0.647～0.984,except for two places with low similarity,the similarity of other places reached more than 0.8.7.The water extract of Psychotria serpens L.had a good inhibitory effect on streptococcus pneumoniae,escherichia coli and staphylococcus aureus,the minimum inhibitory concentration respectively are 0.0625g/m L,0.125g/m L and 0.03125g/m L.Conclusion:The above evaluation methods had good repeatabilit,simple operation,obvious characteristic,which provide reference for the establishment of quality standard of Psychotria serpens L.It lays a foundation for its safe,effective and controllable clinical application. |
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