Effects Of Edaravone And Dexbomeol On The Expression Of Inflammatory Cytokines In The Brain Tissue Of Rat Models Of Intracerebral Hemorrhage

Objective: This experiment studies the effect of edaravone and dexbomeol on the expression levels of TNF-α,IL-1β,and IL-6 in the brain tissue of rat models of intracerebral hemorrhage,and explores whether edaravone and dexbomeol can be the future treatment of cerebral hemorrhage Potential drugs for inflammation in the hind brain tissue.Methods: Take 36 SD female rats(8 weeks,weight of 250 g ～ 300 g)is divided into six groups at random,Namely the SHAM group,the ICH group,edaravone and dexbomeol low dose group(2 m L /kg·d),edaravone and dexbomeol medium dose group(4 m L /kg·d),edaravone and dexbomeol high dose group(6 m L /kg·d)and edaravone group(6 m L /kg·d),each group 6 only.Neurobehavioral tests were performed at 24,48 and 72 hours before and after cerebral hemorrhage,and subcutaneous injection was performed at regular intervals for 3 consecutive days.On the 3rd day after operation,brain tissue of rats in each group was extracted and observed under light microscope with HE staining.The expression levels of TNF-α,IL-1β and IL-6 in brain tissues after intracerebral hemorrhage were detected by enzyme-linked immunosorbent assay.Results:1.HE staining under light microscope showed that the brain tissue and cell level of the rats in the SHAM group were clear,the cells were arranged in an orderly manner,and there was no interstitial edema and inflammatory cell infiltration;compared with the SHAM group,the brain tissue of the rats in the ICH group was loosely arranged,Disorders,necrosis,and obvious interstitial edema and inflammatory cell infiltration;compared with the ICH group,the brain tissue lesions of the rats in each group were significantly improved,which was positively correlated with the drug dose,the number of nerve cell degeneration and necrosis was reduced,and the inflammatory cell infiltration was obvious cut back.2.ELISA showed that the expressions of TNF-α,IL-1β and IL-6 in perihematoma tissues in ICH group were significantly higher than those in Sham group(P < 0.01).Compared with ICH group,the expression of inflammatory cytokines in low-dose group,medium-dose group,high-dose group and edaravone group were significantly different(P < 0.05).Compared with medium dose group,the expression of high dose group was significantly decreased(P < 0.05).Compared with low-dose group,the expression of inflammatory cytokines in medium-dose group was significantly decreased(P < 0.05).The expression levels of TNF-α,IL-1β,and IL-6 decreased with the increase of edaravone and dexbomeol dose,which was negatively correlated with the dose.There was no significant difference in the expression of inflammatory cytokines(P > 0.05)in the contralateral brain(no hematoma).3.The fatigue rotating rod experiment showed that edaravone and dexbomeol effectively improved the recovery of neurological function after cerebral hemorrhage in rats.With the increase of administration time and dose,the dropping time gradually prolonged.Compared with the SHAM group,the ICH group had a significantly shorter drop time of the rat’s rotating rod(P < 0.01);the EDRAVONE group was significantly different from the ICH group(P < 0.01);the HD group was significantly different from the ICH group(P < 0.01);Compared with the LD group,the HD group had a significantly longer drop time(P < 0.05).Conclusions:1.Edaravone and dexbomeol inhibits the expression of TNF-α,IL-1β,IL-6 and the infiltration of inflammatory cells in the brain tissue around the hematoma.2.Edaravone and dexbomeol may promote the recovery of nerve function after cerebral hemorrhage by inhibiting the infiltration of inflammatory cytokines or inflammatory cells.