Dynamic Optical Study Of Cerebral Blood Flow Changes In Cerebral Venous Sinus Throinbosis Model

Cerebral Venous Sinus Thrombosis is a kind of cerebral venous system disease with low incidence and mainly affecting young people.The most patients have a favorable prognosis,but a few patients will continue to increase intracranial pressure or even venous hypertensive intracerebral hemorrhage with the progress of the disease,resulting in more serious symptoms of focal neurological impairment.Therefore,it is very important to further reveal the occurrence and development process of CVST for better understanding of the disease and exploring more effective treatment methods.In this study,laser speckle imaging technology was used to continuously monitor the change trend of cortical blood flow in three groups of mice with different CVST models.Through control analysis,the difference in the influence of different thrombogenic factors on the dynamic change of cortical blood flow in model mice was studied,and the feasibility of continuous observation of cortical blood flow in CVST model mice by laser speckle technology was verified.In this study,mice were selected to make three CVST models:control group,ligation group and application group.Chronic transparent observation windows were made by thinning the skull,and cortical blood flow was monitored by laser speckle imaging.In building preoperative like as a baseline,and postoperative day,postoperative 1 day,3 day,7 day,11 day,14 days after six point observation,again to choose operation area distal proximal vein,bridge veins and cortex microcirculation interested in three areas respectively to area compare with the rate of change in the baseline.In this way,the difference analysis of blood flow changes in different regions in the same experimental group and the difference analysis of blood flow changes in different experimental groups in the same region can be realized.According to the analysis of laser speckle blood flow signal,the difference of overall cortical blood flow value over time was significant in the three groups of model mice.The difference between the control group and the application group was not significant in the regional observation results.The cortical microcirculation blood flow value in the ligation group was higher than that in the distal bridging vein and the proximal bridging vein.The comparison results of different experimental groups in the same observation area showed that there was no significant difference in the blood flow values of distal bridging vein,while the blood flow values of cortical microcirculation and proximal bridging vein,ligation group and affixed group were significantly lower than the control group,and the blood flow values of cortical microcirculation in the ligation group were also significantly higher than that in the affixed group.Vascular imaging analysis under white light images showed that significant progression of vascular proliferation was observed near the operation area in the application group,but not in the ligation group.Endothelial injury of the venous system and changes in the clotting state continue to play an important role in the disease progression after intracranial venous sinus thrombosis.The simple physical obstruction may not be the main cause of the disease progression and the most important problem to be solved.This experiment also verified the feasibility of using laser speckle imaging technology to continuously observe the dynamic changes of cortical blood flow in two CVST model mice for a long time,providing a reliable platform for further study of the corresponding changes in the cortical functional areas of CVST and the influence on the neurovascular coupling mechanism.