The Expression Of Thioredoxin Reductase 1 In Nasopharyngeal Carcinoma And Its Effect On The Biological Behavior Of Nasopharyngeal Carcinoma Cells

Part 1 To screen the core genes of head and neck squamous cell carcinoma and evaluate the clinical prognosisBackground:Head and neck malignant tumors are a difficult problem for otolaryngologists.Identification of new biomarkers is an urgent need for the diagnosis,treatment and prognosis of head and neck malignancies.Method:In our research,the gene expression data and clinical data were downloaded from the The Cancer Genome Atlas(TCGA)database,and the gene chip data set GSE23036 was downloaded from the Gene Expression Omnibus(GEO)database.The two sets of expression data were used to identify differentially expressed genes and WGCNA to clarify gene modules and pivot genes related to head and neck squamous cell carcinoma.Use HAPPI and CytoHubbag data to analyze protein interaction networks.The clinical data of head and neck squamous cell carcinoma from the TCGA database and GEPIA2 were used to analyze the survival rate of pivot genes,and the correlation between pivot genes and tumor stage was further analyzed.Results:2836 and 570 differentially expressed genes(DEGs)were identified in the TCGA database expression data and GEO gene chip data sets,respectively.We found that the green module has the highest correlation with head and neck squamous cell carcinoma,and the pivot genes in the module are also clear.A total of 15 pivot genes were identified.In the Human Protein Atlas(HPA)database,we found that TXNRD1 was overexpressed in head and neck squamous cell carcinoma at the transcription level compared with the normal level of normal tissues,and survival analysis suggested that this gene is a poor prognostic factor for head and neck squamous cell carcinoma.Conclusion:Our results indicate that TXNRD1 has been identified as a potential biomarker and target for head and neck squamous cell carcinoma.Part 2 The effect of TXNRD1 gene on the proliferation,invasion and metastasis of nasopharyngeal carcinoma cellsBackground:In the previous work,we screened TXNRD1 as a potential biomarker for detecting and predicting the prognosis of head and neck squamous cell carcinoma.At present,there is no expression of TXNRD1 in nasopharyngeal carcinoma cells.In order to understand the effect of TXNRD1 gene on the proliferation,invasion and metastasis of nasopharyngeal carcinoma cells,this research work was carried out.Methods:The expression of TXNRD1 in 5-8F and HNE1 cell lines was monitored by RT-PCR and western blot.The experimental group silenced TXNRD1 in 5-8F and HNE1 cell lines,and verified that the gene had been knocked out by RT-PCR and western blot.The control group is 5-8F,HNE1 cell line is not treated.Through CCK-8,transwell experiment to monitor cell migration and invasion ability,flow cytometry analysis of cell cycle and cell apoptosis.Results:1.TXNRD1 showed high expression in 5-8F and low expression in HNE1 cells.2.Among the three targets of TXNRD1(siTXNRD1-1,siTXNRD1-2,siTXNRD1-3),the expression of TXNRD1 decreased most significantly after silencing siTXNRD1-2.3.After silencing the TXNRD1 gene,the migration and invasion ability of nasopharyngeal carcinoma cells decreased,and the apoptosis index increased,and nasopharyngeal carcinoma cells m ainly stagnated in G0/G1 stage.Conclusion:It is worth noting that after bioinformatics analysis and cell language verification,we found that silencing the TXNRD1 gene promoted the apoptosis of nasopharyngeal carcinoma cells.From this,it can be inferred that the TXNRD1 gene is most likely to be the result of nasopharyngeal carcinoma cells.But we still need to further verify it.