Effects Of Sodium Butyrate And Irinotecan On Proliferation And Apoptosis Of Colorectal Cancer Cells

Objective:With the change of modern lifestyle,the incidence of colorectal cancer is increasing year by year.Globally,it has the third highest incidence of all malignancies.Due to the high degree of malignancy and poor prognosis of colorectal cancer,Irinotecan is mainly used for the treatment of advanced colorectal cancer.FOLFIRI regimen commonly used in patients with metastatic colorectal cancer includes Irinotecan,5-fluorouracil and calcium leucovorin.Although the drug is effective,there are great differences in efficacy and safety among patients,and a considerable proportion of patients have serious adverse reactions due to drug toxic and side effects.Therefore,the combination of effective low-toxicity drugs with chemotherapy drugs with different mechanisms of action is a feasible way to further improve the efficacy and reduce toxic and side effects.Sodium butyrate,as a Histone deacetylase(HDAC)inhibitor,has been shown to inhibit tumor cell proliferation and promote apoptosis in various malignant tumors.Sodium butyrate is the final product of dietary fiber decomposition by bacteria in the colon.Its synergistic anti-tumor effect with irinotecan is worthy of further study.In this study,the effects of sodium butyrate and irinotecan monotherapy and combined administration on proliferation and apoptosis of colorectal cancer cell lines HCT116,SW480 and Lo Vo were investigated.Methods:Three different colorectal cancer cells HCT116,SW480 and Lo Vo were selected.Cck-8 method was used to detect the effects of sodium butyrate and irinotecan at different concentrations single drug or combination on the proliferation of colorectal cancer cells,respectively.According to the results of CCK-8 experiment,sodium butyrate with concentration of 5mmol/L and irinotecan with concentration of40μmol/L were selected for combined action.Flow cytometry was used to detect the effect of single drug and combined drug on the apoptosis of colorectal cancer cells.Results:Both sodium butyrate and irinotecan inhibited the proliferation and apoptosis of HCT116,SW480 and Lo Vo cells in concentration dependence and time dependence(P <0.05).Compared with single drug treatment,sodium butyrate combined with irinotecan on HCT116,SW480 and Lo Vo cells could significantly inhibit cell proliferation and promote apoptosis(P <0.05).Conclusion:1.Sodium butyrate and irinotecan can inhibit proliferation and promote apoptosis in colorectal cancer cells.2.The combination of sodium butyrate and irinotecan can enhance the inhibitory effect on the proliferation of colorectal cancer cells and induce cell apoptosis,and sodium butyrate has a synergistic effect with the chemotherapy drug irinotecan.