| Objective:This thesis aims to analyze the level of S100B protein in the peripheral blood of preterm infants of different gestational ages,and explore the difference of S100B protein in the serum of preterm infants of different gestational ages,so as to evaluate its diagnostic value.Based on the amplitude of the children’s amplitude integrated electroencephalogram(a EEG),head B-ultrasound and head magnetic resonance imaging(Magnetic Resonance Imaging,MRI)and other examination results,brain injury group and no brain injury group are divided.This thesis analyzes the difference of serum S100B protein level in each group,and provides evaluation indicators for early clinical evaluation of brain injury in premature infants after asphyxia.Methods:A total of 117 cases of asphyxiated preterm infants who were born in the Taizhou People’s hospital from October 2018 to October 2020 were collected(25 cases in the very preterm infant group,38 cases in the moderate preterm infant group,and 54 cases in the late preterm infant group).According to a EEG,cranial ultrasound and cranial MRI,35 cases of brain injury group and 82 cases of no brain injury group were divided.In addition,121 cases of non-asphyxia preterm infants born in the same hospital were collected(24 cases of very preterm infants,36 cases of moderate preterm infants),61 cases in the late preterm infant group),the serum S100B protein level of preterm infants was measured by enzyme-linked immunosorbent assay(ELISA).Results:1.The serum S100B protein level in the observation group was(0.77±0.32)μg/L,and the serum S100B protein level in the control group was(0.51±0.23)μg/L.The difference between the two groups was statistically significant(t=7.357,P<0.001).Very premature infants in the observation group: mild asphyxia(0.85±0.21)μg/L,severe asphyxia(1.35±0.31)μg/L,the difference between the two groups was statistically significant(t=4.754,P<0.001);moderate preterm birth Infants: mild asphyxia(0.74±0.18)μg/L,severe asphyxia(1.16±0.27)μg/L,the difference between the two groups was statistically significant(t=5.369,P<0.001);late preterm infants:mild asphyxia(0.52±0.13)μg/L,severe asphyxia(1.02±0.21)μg/L,the difference between the two groups was statistically significant(t=9.465,P<0.001).2.Observation group: The serum S100B protein level in the very preterm infant group was(1.05±0.35)μg/L,the moderate preterm infant group was(0.83±0.26)μg/L,and the late preterm infant group was(0.61±0.24)μg/L.The difference between the groups was statistically significant(F=23.897,P<0.001).Serum S100B protein level was negatively correlated with gestational age(r=-0.085,P<0.001).Control group:The serum S100B protein level in the very preterm infant group was(0.84±0.16)μg/L,the moderate preterm infant group was(0.52±0.14)μg/L,and the late preterm infant group was(0.37±0.14)μg/L.The difference between the groups was statistically significant(F=90.863,P<0.001).Statistical analysis by linear regression equation showed that serum S100B protein level was negatively correlated with gestational age(r=-0.081,P<0.001).3.In the brain injury group,the average level of serum S100B protein in preterm infants was(1.21±0.24)μg/L,and in the non-brain injury group,the average level of serum S100B protein in preterm infants was(0.63±0.18).)Μg/L.The difference between the two groups was statistically significant(t=13.929,P<0.001).If analyzed according to different gestational age groups,the serum S100B protein level of the very preterm infants,moderate preterm infants,and late preterm infants in the brain injury group was higher than that of the non-brain injury group,and the difference between the groups was statistically significant(P<0.001).Conclusion:1.Serum S100B protein level in premature infants with asphyxia significantly increased,and with the severity of asphyxia,the level of S100B protein showed corresponding changes,suggesting that S100B protein is related to the severity of asphyxia in preterm infants.2.There was a negative correlation between gestational age and the serum S100B protein level of preterm infants.The smaller the gestational age,the higher the serum S100B protein level,suggesting that gestational age is a factor affecting the serum S100B protein level of preterm infants.3.The serum S100B level of preterm infants with brain injury was higher than that of preterm infants without brain injury,and the level of serum S100B protein in preterm infants with brain injury of different gestational ages was higher than that of preterm infants without brain injury at the same gestational age,suggesting that S100B protein can be used as a serological indicator of the severity of brain damage in premature infants. |
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