Study On The Safety Of Rectal Cancer Margin Based On Liquid Chromatography-mass Spectrometry

Objective:Our study used metabolomics way explore the different metabolites between the tumor tissue and normal tissue in rectal tumor patients.And then compared the relative abundance with the distance of the cutting margin.In order to assesment the accuracy margin of the rectal tumor.To provide the theoretical basis for the colinical practice.Method:A total of 150 tissue samples(6 tissue samples from each patient)were collected from 25 patients with rectal cancer(5 patients with T1,T2 and 20 patients with T3,T4)by liquid chromatography-mass spectrometry(LC-MS).1group tumor center tissue,2 group tissue0.5cm,3 group 1cm,4 group 2cm,5 group 3cm,6 group 5cm from the tumor)were detected respectively,and their metabolic profiles and data were obtained.First,the TIC and PCA results of the tumor center and the samples 5cm away from the tumor(normal tissue)were compared to observe whether there was any difference between each group.After that,different metabolites were screened out through various analysis methods.For the above product specificity in T1,T2 and T3,T4 aof group 1,2,3,4,5,6 add 6 groups of clustering analysis,according to the results of cluster analysis is to group the discriminant analysis and then further,further screening,and then by comparing the 1,2,3,4,5,6 six changes in abundance in the group,to accurately distinguish between tumor and normal tissue,To provide a theoretical basis for the accurate judgment of the safety of surgical margin.Result:(1)Metabonomics analysis was conducted on the tumor center tissues of patients with rectal cancer and the normal tissues 5cm away from the tumor,and the following conclusions were obtained:Ethyl 3-Hydroxy Triester,Ditriglyceride,7-Dehydrocholine,Acetaminophenylalamine,Triglyceride Monocelate,24-O-B-D-Glycoside,Triglyceride,N-Ethanolamine,Propylcarnitine,Fatty Acetyl,Fatty Alcohol,3,4-Dihydrooxyphenylpropanol,4,5 Epoxy amide,4-hydroxybutadibenzoic acid,3’-phosphoamine sulfate 5’-phosphate,1-O-β-Dglucosol-2,1-acetylglycerol phosphate,1,2,3-triacetylglycerol,Amphetamine nucleoside monophosphate,adenosine acid,N-acetyl-α amino acid,N-acrylamide are specific metabolites to distinguish between rectal cancer and normal tissue.(2)Patients with T1 and T2 stage rectal cancer:Acetaminophenilic Acid,Ethyl 3-Hydroxy Triester,1-Acetylglycerin Phosphoric Acid,1-Acetylglycerin Nucleoside Monophosphate,1-Acetylglycerin Phosphoric Acid,N-Acrylamide,4-Hydroxy Butanedibenzoic Acid,4,5.The relative abundances of epoxylamide,adenylate,7-dehydrocholine,and propylcarnitine changed most significantly from the tumor center to the normal tissue 0.5cm away from the tumor,and the relative abundances showed a stable trend in the subsequent tissues.(3)In patients with rectal cancer in T3 and T4 stage:Acetyl amino benzedrine acid,ethyl3-1-acetyl glycerin three esters,phosphoric acid,adenosine,benzedrine nucleoside monophosphate,1-b glycerol phosphate,N-acrylamide relative abundance from the cancer center to normal)relative abundance changes at 2 cm tumor and most obvious,and the relative abundance in the stable trend in the organization change.Conclusion:(1)There are differences in metabolites between rectal cancer tissues and normal tissues,and these products may be used as differential markers for the diagnosis of rectal cancer.(2)In this study,0.5cm in T1 and T2 stage rectal cancer patients had the same mass spectrum results as normal tissue,and the safe boundary distance of tumor differentiated by different metabolites was 0.5cm.(3)In this study,2cm in patients with T3 and T4 stage rectal cancer had the same mass spectrum results as normal tissue,and the safe boundary distance of tumor differentiated by different metabolites was 2cm.