Chemical Compositions And Biological Activities Of Cryptoporus Sinensis And Pinellia Ternata

This thesis mainly includes the following two aspects of research content.In the first chapter,the chemical constituents of C.Sinensis was studied.In the second chapter,the chemical constituents and in vitro anti-tumor activity screening of P.ternate were studied.And the research progress of genus Cryptoporus in the past thirty years was summarized in the last part.Objective:To study the chemical compositions of C.Sinensis and P.ternate,and searched for new anti-tumor active substances through in vitro cytotoxic activity.Methods:The compounds were isolated and purified by using various column chromatography methods including silica gel,Rp-18,Sephadex LH-20,MCI and Preparation of thin layer chromatography.1D,2D NMR,HR-ESI-MS,UV and IR spectral analysis were used to identify the structure of the compound.Furthermore using MTS method to study anti-tumor activity of new componds.Results:In the experiment,95% ethanol was used for extraction,followed by extraction with petroleum ether,ethyl acetate and n-butanol.Twenty-three compounds were obtained from Petroleum ether and ethyl acetate part of 95% ethanol extract of fruit body of C.Sinensis,and identified as Cryptoporic acid A methyl ester(1),Cryptoporic acid B(2),Cryptoporic acid B methyl ester(3),Cryptoporic acid D(4),Cryptoporic acid D dimethyl ester(5),Cryptoporic acid E(6),Cryptoporic acid E methyl ester(7),Cryptoporic acid E dimethyl ester(8),Cryptoporic acid G(9),Cryptoporic acid J(10),Cryptoporic acid L(11),Cryptoporic acid N(12),11-hydroxyldrim-8,12-en-14-oic acid(13),Ergosterol(14),5α,8α-peroxyergosta-6,22-diene-3β-ol(15),Ergosta-7,22-dien-3-one(16),trametenolic acid B(17),5α,6α-epoergoster-8(14),22-diene-3β,7α-diol(18),Ergosta-7,22-diene-3β,5α,6β,9α-tetraol(19),Ergosta-7,22-diene-3β,5α,6β-triol(20),Octadecadienoic acid(21),Palmitic acid(22),Glyceryl monopalmitate(23).Nine compounds were obtained from ethyl acetate part of 95% ethanol extract of Tuber of P.ternate,and identified as Pinternatines A(1),Pinternatines B(2),(2S,3S,6S)-2-methyl-6-(9-phenylnonyl)piperidin-3-ol(3),Ephedrine(4),P-hydroxycoumaric acid(5),Chrysophanol(6),Stigmasterol(7),β-sitosterol(8),Linoleic acid-1-monoglyceride(9).Among them,compounds 1 and 2 were two new organic amine compounds,and the anti-tumor active test results show that both compounds 1 and 2 have good cytotoxic activity.Conclusion:The chemical constituents were mainly cryptoporous acid and steroids of C.Sinensis and several cryptoporous acid had been reported with anti-tumor,anti-inflammatory and antioxidant activities.It were Alkaloids containing that isolated from P.ternate,In vitro cytotoxic activity screening found that both compounds 1 and 2have good cytotoxic activity on experimental tumor cell lines,which verifies the rationality of P.ternate anti-tumor effect.