The Expression And Biological Function Of HIST1H3B In Colorectal Cancer

Objective:This study explored the differential expression of HIST1H3B between colorectal cancer and adjacent normal tissues by bioinformatics technology,two-dimensional chromatography and mass spectrometry and immunohistochemistry.To explored the biological function of the differential expression of HIST1H3B in CRC through the cell function experiment,which provided a potential molecular target for the diagnosis and treatment of colorectal cancer.Methods:1.We determined that HIST1H3B is up-regulated in colon cancer by using public database Timer.Download the gene expression profile data of colon cancer and normal colon tissue through the TCGA database,and analyze the data to determine the expression of HIST1H3B in colon cancer.2.The expression of HIST1H3B in CRC and adjacent normal tissues was detected in 34 pairs of tissues by two-dimensional chromatography and mass spectrometry and 47 pairs of tissues by immunohistochemistry.3.Overexpression and silenced of HIST1H3B were constructed in CRC cell lines SW1116 and SW620,and we used RT-qPCR and Western Blot to detect the transfection efficiency.The cell function experiments were used to explore the effect of HIST1H3B expression level on the ability of proliferation,migration and invasion of CRC cells.Results:1.HIST1H3B is significantly up-regulated in colon cancer and other cancer.2.Two-dimensional chromatography and mass spectrometry technology shows that HIST1H3B is up-regulated in CRC.The results of immunohistochemical verify that HIST1H3B is up-regulated in colorectal cancer。3.RT-qPCR and western blot analysis determined that we successfully constructed HIST1H3B overexpressed and silenced cell lines.After overexpression HIST1H3B,cell function experiments show that the ability of proliferation,migration and invasion of CRC cells was promoted.After silencing HIST1H3B,the proliferation,migration and invasion of CRC cells was inhibited.Conclusions:1.HIST1H3B is up-regulated in colorectal cancer tissues.2.The up-regulated expression of HIST1H3B can promote the ability of proliferation,migration and invasion of colorectal cancer cells.This gene might be a potential tumor biomarker for diagnosis and treatment of CRC.