| PurposeIn this study,the Cancer Genome Atlas(TCGA),Genotype-Tissue Expression,GTEX database and a single-center retrospective cohort of non-small Cell Lung Cancer(NSCLC)were analyzed.The correlation between the expression of AMSH(Associated Molecule with the SH3 Domain of STAM),clinical variables and OS(Overall Survival)and disease-free Survival(DFS)were analyzed further.The study might provide a new potential target for the diagnosis,treatment and prognosis of patients with non-small cell lung cancer.MethodsThe differential expression of AMSH in cancer tissues and para-cancerous tissues of multiple tumor species was firstly analyzed and predicted through GEPIA data platform.And then the correlation between AMSH expression and OS and DFS of patients with NSCLC was further explored,as well as its relationship with EGFR(Epidermal Growth Factor Receptor)expression.A total of 56 cases of paraffin specimens of cancer tissues and corresponding para-cancerous tissues were collected from patients who underwent radical resection of lung cancer in a Grade III A hospital from January,2012 to December,2014,which included 36 male patients and 20 female patients.During the follow-up,5 patients were lost to follow-up,and 51 patients were finally included in the statistics.Immunohistochemical detection using rabbit anti-human AMSH-N-Terminal polyclonal antibody was performed in paraffin sections of non-small cell lung cancer and para-cancerous tissues of the selected patients.After staining,the sections were independently interpreted by two qualified pathologists by double-blind method.If the interpretation results of two people were inconsistent,the disputed specimens would be re-interpreted by a third physician,respectively.Univariate and multivariate statistical analysis methods of Cox proportional risk model were used to analyze the effects of various factors on OS and PFS.p<0.05 mean the influencing factors had statistical significance.Results1.The results of GEPIA platform prediction showed that AMSH was highly expressed in lung squamous cell carcinoma tissues,compared with para-cancerous tissues(p<0.05).And the high expression of AMSH in lung squamous cell carcinoma tissues was negatively correlated with the patients’ DFS(p=0.027).There was no statistical significance with the patients’ OS(P =0.068).In lung adenocarcinoma tissues,the high expression of AMSH was negatively correlated with the patients’ OS(p=0.010),and no statistical significance with the patients’ DFS was observed(p =0.600).2.Immunohistochemical results of NSCLC tissues and para-cancerous tissues showed that AMSH was highly expressed in 33 of 51 NSCLC specimens(64.7%);Low expression was observed in 18 cases(35.3%).In the corresponding 51 cases of para-cancerous tissues,46 cases showed low expression and 5 cases showed high expression of AMSH.Chi-square test showed that the difference was statistically significant(p =0.001).3.Relationship between AMSH expression and clinicopathological characteristics in patients with lung cancer showed that AMSH expression in tumor cells was significantly correlated with T stage,lymph node metastasis,and tumor cell differentiation degree(p<0.05),but had no significant correlation with age,sex,smoking and other factors(p>0.05).4.Correlation of AMSH expression and clinicopathological features with OS and PFS revealed that univariate analysis of Cox proportional model showed significant correlation between AMSH expression and DFS(p =0.001),but no correlation with OS(p=0.135).The median OS of AMSH positive group was 82.70 months and the median DFS was 68.50 months.The median OS and DFS in the groups with negative AMSH expressions had not been reached at the end of follow-up and analysis.T staging was significantly correlated with OS(p=0.042,p=0.002,p=0.004,respectively)and DFS(p=0.024,p=0.001,p=0.005,respectively).Lymph node metastasis(p=0.022,p=0.021,respectively)was also significantly correlated with OS and DFS.Other factors,such as degree of differentiation,age,sex,smoking or not,had no significant correlation with OS and DFS(p>0.05).Multivariate analysis results of Cox proportional model showed that T staging was significantly correlated with OS and DFS,and lymph node metastasis was also correlated with OS.The analysis was statistical significance.The expression of AMSH and other factors had no correlation with OS and DFS.Conclusions1.In non-small cell lung cancer,the expression of AMSH in tumor tissues was significantly different from that in para-cancerous tissues.2.The expression of AMSH is correlated with T stage,lymph node metastasis and tumor cell differentiation degree.3.The high expression of AMSH affects the prognosis of NSCLC,but it is not an independent prognostic factor for NSCLC.Cox univariate analysis results showed that patients with high AMSH expression had shorter time of DFS,while patients with late T staging and regional lymph node metastasis had shorter OS and DFS.The differences were statistically significant.Cox multivariate model analysis showed that T stage and lymph node metastasis were independent predictors for OS and DFS of NSCLC. |
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