| Background:Acne is a common chronic inflammatory disease of the hair follicles and sebaceous glands,which usually occurs in adolescents.Due to its chronic course,sometimes severe and sometimes light,easy to leave pigmentation or scars,which seriously affects the physical and mental health of patients,making it a physical,psychological,and social problem faced by the whole society.Acne has a high incidence in adolescents.Clinically used methods for the treatment of acne include topical use of tretinoin,benzoyl peroxide,oral isotretinoin,antibiotics,and photodynamic.However,these methods have many shortcomings such as dry desquamation,long treatment time,poor compliance,drug resistance,and severe inflammation.Therefore,seeking better treatments has always been a hot spot in scientific research.In recent years,photothermal therapy(PTT)has attracted much attention as a new potential acne treatment.PTT can selectively act on the sebaceous glands,inhibiting the proliferation of sebaceous glands and the secretion of sebum.Au-Ag nanoparticles(NPs)have excellent photothermal conversion efficiency after absorbing near-infrared light,making them an excellent material for PTT.Polydopamine is used as a coating to make Au-Ag NPs have better stability and biocompatibility.In addition,the use of cell membrane camouflage nanoparticle technology to encapsulate Au-Ag-PDA NPs can increase the uptake of NPs by cells,improve their photothermal conversion efficiency,and reduce the cytotoxicity of NPs.Umbilical cord mesenchymal stem cells have become a favorable source of cell membrane drug-carrying systems due to their easy availability and low immunogenicity.This article discusses the safety and effectiveness of photothermal therapy mediated by Au-Ag-PDA NPs(Au-Ag-PDA@STCM)encapsulated in mesenchymal stem cell membrane(STCM)in vivo and in vitro.Then verify the potential value of NPs in the treatment of acne.Objectives:Observe the effect of Au-Ag-PDA@STCM-guided photothermal therapy on sebaceous gland tissue and sebaceous gland cells in vivo and in vitro experiments.Explore the role of Au-Ag-PDA@STCM-guided photothermal therapy in the treatment of acne application prospects.Methods:1.In vitro experiment: The human sebaceous gland cell line SZ95 was used to conduct the study of the nanoparticle in vitro photothermal experiment.SZ95 cells were treated with nanoparticles and were irradiated with near-infrared(NIR)laser.The CCK8 method was used to detect the cell survival rate.By changing the concentration of NPs,laser irradiation time and laser energy density,the effect of Au-Ag-PDA@STCM NPs on SZ95 cell proliferation was explored.2.In vivo experiment: The golden hamster,an animal model of acne inflammation,was used to observe the photothermal therapeutic effect of Au-Ag-PDA@STCM NPs in vivo.Apply different concentrations of Au-Ag-PDA NPs and Au-Ag-PDA@STCM aqueous solutions on the flank organs of golden hamsters,and then irradiate them with 808 nm NIR laser.Immediately after treatment,10 days after treatment,20 days after treatment,and 30 days after treatment,tissue samples of flank organs were taken for H&E staining and oil red O staining at these 4time points.By observing the sebaceous gland size and sebum secretion changes of golden hamsters,the photothermal therapeutic effect of Au-Ag-PDA@STCM NPs on sebaceous gland tissue in vivo was verified.3.Results:1.In vitro experiment: The photothermal effect of NPs was observed.When the concentration of NPs was changed,the cell survival rate of human sebaceous gland cell line SZ95 decreased with the increase of NPs concentration.And the anti-proliferative effect of Au-Ag-PDA@STCM NPs is stronger than that of Au-Ag-PDA NPs.When the laser irradiation time is changed,the cell survival rate of SZ95 decreases with the extension of the laser irradiation time,and the result of the stronger anti-proliferation effect of Au-Ag-PDA@STCM NPs can also be obtained.When the laser energy density is changed,the cell survival rate of SZ95 decreases with the increase of the laser energy density,and the anti-proliferation effect of Au-Ag-PDA@STCM NPs is stronger.The results of in vitro experiments showed that NPs significantly inhibited the proliferation of SZ95 cells in a concentration-dependent,laser-irradiated time-dependent and laser-energy-density-dependent manner,and Au-Ag-PDA@STCM NPs had better anti-proliferation effects than Au-Ag-PDA NPs.2.In vivo experiment: The photothermal treatment effect mediated by Au-Ag-PDA@STCM NPs and Au-Ag-PDA NPs was continuously observed for 30 days.Histological analysis of the flank organs of golden hamsters immediately after photothermal treatment showed that the sebum secretion of the sebaceous glands of the two groups of golden hamsters was significantly reduced.After 10 days of photothermal treatment,histological analysis showed that the sebaceous glands of the two golden hamsters were significantly reduced and the sebum secretion was significantly reduced,but there was no significant difference between the two groups.The photothermal effect of Au-Ag-PDA@STCM NPs and Au-Ag-PDA NPs showed a difference after 20 days,and the photothermal effect of Au-Ag-PDA@STCM NPs was better than that of Au-Ag-PDA NPs.In vivo experimental results show that the photothermal effect mediated by Au-Ag-PDA@STCM NPs significantly reduces sebaceous glands and inhibits sebum secretion,and the photothermal treatment of Au-Ag-PDA@STCM NPs is significantly better than Au-Ag-PDA NPs.Conclusions:Au-Ag-PDA@STCM NPs-mediated photothermal treatment inhibits the proliferation of SZ95 cells in vitro,significantly reduces sebaceous glands and reduces sebum secretion in vivo.This method can provide new ideas for the treatment of acne,and has a great prospect of clinical application in the future. |
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