Expression And Significance Of Stromal Cell-derived Factor-1 And Its Receptor CXCR4 In Fibroblasts From Pterygium

Objective: To study the expression of stroma cell-derived factor-1(SDF-1)and its receptor(C-X-C chemokine receptor 4,CXCR4))in fibroblasts of pterygium and its significance.Methods: Primary pterygium and normal conjunctival tissue were cultured and divided into 4 groups according to age> 50 years old and ≤50 years old.Pterygium group A: age≤50 years old group,group B: age>50 years old group.Normal conjunctiva group: C group:age ≤50 years old;D group: age > 50 years old.The expression of SDF-1 and its receptor CXCR4 in pterygium fibroblasts and normal conjunctival fibroblasts from patients with different ages were detected by immunofluorescence staining,Western-blot ELISA and real-time quantitative PCR,and their significance was analyzed.Results: In normal conjunctival fibroblasts,the m RNA expressions of SDF-1 and CXCR4 in group C were not significantly different from those in group D(P > 0.05).In pterygium fibroblasts,the protein and m RNA expressions of SDF-1 and CXCR4 in group≤50 years old were higher than those in group > 50 years old(P < 0.05).Conclusion: SDF-1/CXCR4 may be involved in the occurrence and development of pterygium,and it may be related to the high recurrence of pterygium in people under 50 years old.The inhibition or down-regulation of SDF-1/CXCR4 expression may be a new target for the prevention of pterygium recurrence.