Effects Of MiRNA-107 On Proliferation,Migration And Invasion Of Cervical Cancer Cells By Targeting HMGB1 And Its Mechanism

ObjectiveTo investigate the effect of miR-107 on the proliferation,invasion and apoptosis of cervical cancer cells through targeted HMGB1 and its molecu lar mechanism.MethodsThe normal cervical cell（Ect1/E6E7）and cervical cancer cell line（C4-1、Si Ha、Caski、He La）were collected and cultured at 37℃and 5%CO_2。Cervical cancer C4-1 cells and He La cells were divided into control group,miR-NC group,miR-107 mimics group,miR-107 inhibitor group,miR-107 inhibitor+si-HMGB1group.Quantitative real-time polymerase chain reaction（q RT-PCR）was used to detect the expression of miR-107,Ki-67,E-cad,N-cad.The proliferation of cancer cells was detected by CCK-8 method.The invasion of cancer cells was measured by Transwell experiment.The apoptosis of cancer cells was examined by flow cytometry,and the expression levels of proteins were tested by Western blot.Double luciferase reporter assay was used to detect the targeting relationship between miR-107 and HMGB1.ResultsCompared with the normal cervical cell,the expression level of miRNA-107in cervical cancer cell lines was significantly decreased,and the differences were statistically significant（P<0.05）.The miRNA-107 level in the miR-107mimics group transfected with miR-NC mimics was significantly higher than that in miR-NC group,and the differences were statistically significant（P<0.05）.Upregulation of miR-107 inhibited the proliferation and invasion,decreased the m RNA expression levels of Ki-67,increased the apoptosis of C4-1 cells and He La cells,increased the m RNA expression level of E-cad,and reduced the m RNA expression level of N-cad,and the differences were statistically significant（P<0.05）.miR-107 targeted the expression of HMGB1.After interfering with miR-107 inhibitor,the cell proliferation and invasion increased,the apoptosis decreased,E-cad m RNA level decreased,and N-cad m RNA level increased;compared with miR-107 inhibitor group,co-transfection with miR-107inhibitor and si-HMGB1 inhibited cell proliferation,invasion,and N-cad m RNA level,promoted cell apoptosis and E-cad m RNA level.ConclusionsmiRNA-107 in cervical cancer cells in vitro was low expression.The overexpression of miRNA-107 can significantly inhibit cell proliferation and invasion,promoted cell apoptosis by targeting with HMGB1,which will provide a new idea of clinical cervical cancer treatment and potential new target for cervical cancer clinical intervention.